Abstract
In this work, the enantioselective bioaccumulation and dissipation of uniconazole enantiomers in earthworm-soil microcosm were studied. A fast enantioseparation method of uniconazole through supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) was established. The CHIRALCEL OZ-3 column and a mixture of CO2 and methanol (80:20, v/v) were used within 1.0 min to separate uniconazole enantiomers. The recoveries of uniconazole enantiomers in earthworm and soil samples ranged from 83.3 to 113%, and the intra-day and inter-day relative standard deviation values were lower than 11%. In earthworms, the bioaccumulation concentrations of uniconazole enantiomers increased with time and reached the maximum on the 7th day and then decreased. The elimination of uniconazole enantiomers in earthworms followed the first-order kinetics equation, and the elimination half-lives were approximately 7 days. In artificial soil, the dissipation of uniconazole enantiomers was slow, and the dissipation half-lives were both 25.7 days. No enantioselectivity occurred in the earthworm-soil microcosm. These results may reduce the uncertainty of environmental risk assessment for uniconazole.
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Funding
This study was supported by the Science Technology Project of Zhejiang Province [Grant No. 2020C02023], the National Natural Science Foundation of China [Grant No. 22006137], and the Natural Science Foundation of Zhejiang Province [Grant No. LQ21C140002].
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Chunhong Teng: investigation, roles/writing—original draft, validation, and visualization. Ying Li: conceptualization, roles/writing—original draft, and methodology. Tao Cang: resources, data curation, and methodology. Hao Xu: investigation and data curation. Zhenzhen Liu: software and supervision. Peipei Qi: investigation. Zhiwei Wang: formal analysis and resources. Huiyu Zhao: methodology. Shanshan Di: investigation, resources, project administration, and writing–review and editing. **nquan Wang: resources, project administration, and writing—review and editing. The authors read and approved the final manuscript.
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Teng, C., Li, Y., Cang, T. et al. Study on the enantioselective bioaccumulation and dissipation of uniconazole enantiomers in earthworm-soil microcosm through supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS). Environ Sci Pollut Res 30, 29432–29441 (2023). https://doi.org/10.1007/s11356-022-24023-3
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DOI: https://doi.org/10.1007/s11356-022-24023-3