Abstract
Electromagnetic fields (EMFs) are common in our everyday lives. They have many origins and severe effects on individuals and environments where they inflict a great deal of health and psychological harm. The current study investigated the impact of high voltage (H.V.) EMF 5.4 kV/m for 2 and 4 h per day with a frequency equal to 50 Hz alternating current (AC) on body weight (b.wt), blood indices, and certain liver enzymes of albino rats after 25 days of exposure to the electromagnetic field. This work focuses on the therapeutic action of methanol extract of Rosmarinus officinalis (R. officinalis) leaves at a dose (5 mg/kg b. wt) against harmful EMF-induced effects. The findings showed that electromagnetic field exposure induced a substantial decrease in red blood cells (RBC), haemoglobin concentration (Hb), and catalase activity (CAT). Although white blood cells (WBCs), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, urea, creatinine, uric acid, and malondialdehyde (MDA) levels have increased significantly under EMF treatment. Treatment with R. officinalis showed attenuation in these parameters that were induced in rats exposed to H.V. These findings were followed by the histopathological analysis of the liver in the observations. Finally, we conclude that R. officinalis leaves extract offered substantial protection against H.V-induced liver damage and can be applied in drug production.
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References
Abd El Kader AM, El Sammad MN, Taha H (2012) The protective role of rosemary (Rosmarinus officinalis) in lead acetate induced toxicity in rats. J Applied Sci Res 8(6):3071–3082
Abd El Rahman NA, Abd El Hady AM, El Tahawy NA (2014) Silymarin and vitamin E modulates 900MHz electromagnetic field induced oxidative stress and hormonal changes in male albino rats. J Amer Sci 10(9):1–8
Achudume AC, Onibere B, Nwoha P, Alatise O, Aina B (2012) Effects of radiation emitted from base stations on bilirubin, transaminases and lipid peroxidation in exposed rats. Energy Environ Res 2(1):1–10
Aebi H (1984) Catalase in vitro. Methods Enzymol. Academic Press. 105: 121-126
Amara S, Abdelmelek H, Salem MB, Abidi R, Sakly M (2006) Effects of static magnetic field exposure on hematological and biochemical parameters in rats. Braz Arch Biol Technol 49(6):889–895
Aweda MA, Gbenebitse SO, Kehinde MO (2004) Effects of 2.45 GHz radio frequency exposures on normal and sickle cell erythrocytes. Nig J Health Biomed Sci 3(1):56–59
Ayoola GA, Coker BAH, Adesegun AS, Adepoju-Bello AA, Obaweya K, Ezennia CE, Atangbayila OT (2008) Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in south western Nigeria. Tropical J Pharmaceutical Res 7(3):1019–1024
Bancroft JD, Stevens A, Turner DR (1996) Theory and practice of histological techniques. Churchil Livingstone, New York, London, San Francisco, Tokyo. 129–134
Barham D, Trinder P (1972) Determination of uric acid level. Analyst. 97(1151):142–145
Bartles H, Bohmer M, Heirli C (1972) Determination of creatinine level. Clin Chem Acta 37:161
Berrahal AA, Lasram M, Elj NE, Kerkeni A, Gharbi N, El-Fazaa S (2011) Effect of age-dependent exposure to lead on hepatotoxicity and nephrotoxicity in male rat. Environ Toxicol 26(1):68–78
Brand- Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28(1):25–30
David JM, Barreisors NJ, Povid JP (2004) Antioxidant phenyl propanoid enters of triterpenes from Dioclealasiophylla. Pharm Biol 42(1):36–38
Đinđić B, Sokolović D, Krstić D, Petković D, Jovanović J, Muratović M (2010) Biochemical and histopathological effects of mobile phone exposure on rat hepatocytes and brain. Acta Medica Mediterr 49(1):37–42
Doumas BT, Watson WA, Biggs HG (1971) Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 31(1):87–96
Ebrahimzadeh MA, Pourmorad F, Bekhradina AR (2008) Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol 7(18):3188–3192
Edamatsu R, Mori A, Fujita Y, Yasuhara E (1989) Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chem Pharm Bull 7:2016–2021
Edrah SM, Alafid F, Imramovský A, Altwair K, Alkhumsi SI, Hrdina R (2017) Phytochemical screening and antibacterial activity of genista microcephala and Rosmarinus officinalis extracts from libyan’s regions. Int J Res Ayurveda Pharm 8(4):52–56
Eid AF, El-Gendy MA, Zahkouk AS, El-Tahway AN, El-Shamy AS (2015) Ameliorative effect of two antioxidants on the liver of male albino rats exposed to electromagnetic field. Egypt J Hosp Med 58:74–93
El-Bediwi A, El-kott AF, Saad M, Eid E (2011) Effects of electromagnetic radiation produced by mobile phone on some visceral organs of rat. J Med Sci 11(6):256–260
El-sherif MN, Issa MN (2015) Protective effect of rosemary (Rosmarinus officinalis) extract on naphthalene induced nephrotoxicity in adult male albino rat. J Interdiscip Histopathol 3(1):24–32
Emre M, Cetiner S, Zencir S, Unlukurt I, Kahraman I, Topcu Z (2011) Oxidative stress and apoptosis in relation to exposure to magnetic field. Cell Biochem Biophys 59(2):71–77
Fawcett JK, Scott JE (1960) Determination of urea by method using Berthelot reaction. J Clin Pathol 13(2):156–159
Ganong WF (1999) Energy balance, metabolism and nutrition. Rev Med Physiol 22:465
Gazwi HSS, Mahmoud ME, Hamed MM (2020) Antimicrobial activity of rosemary leaf extracts and efficacy of ethanol extract against testicular damage caused by 50-Hz electromagnetic field in albino rats. Environ Sci Pollut Res 27:15798–15805. https://doi.org/10.1007/s11356-020-08111-w
Ghasemzadeh A, Khaki A, Farzadi L, Khaki A, Marjani M, Ashteani HA, Ouladsahebmadarek E (2011) Effect of rosmarinic acid on estrogen, FSH and LH in female diabetic rats. Afr J Pharm Pharmacol 5(11):1427–1431
Gibbs RD (1974) Chemotaxonomy of flowering plants. McGill queen’s university press, Montreal & London. l(1)
Gîrd EC, Nencu I, Popescu LM, Costea T, Duţu EL, Balaci DT, Olaru TO (2017) Chemical, antioxidant and toxicity evaluation of rosemary leaves and its dry extract. Farmacia 65(6):978–983
Gornall AG, Bardwill CS, David MM (1949) Determination of serum proteins by means of biuret reaction. J Biol Chem 177(2):751–766
Grigor’ev IG, Mikhaĭlov VF, Ivanov AA, Mal’tsev VN, Ulanova AM, Stavrakova NM, Nikolaeva IA, Grigor’ev OA (2010) Autoimmune processes after long-term low-level exposure to electromagnetic fields (the results of an experiment) Part 4 Manifestation of oxidative intracellular stress-reaction after long-term non-thermal EMF exposure of rats. Radiats Biol Radioecol 50(1):22–27
Guler G, Turkozer Z, Tomruk A, Seyhan A (2008) The protective effects of N- acetyle- L- cystiene and epigallocatechin- 3- gallate on electric field induced hepatic oxidative stress. Int J Radiat Biol 84(8):669–680
Hamzaa RG, El Shahat AN, Mekawey HMS (2012) Modulating efficiency of γ-irradiated Rosemary in improving the hepatic antioxidant status of ethanol administrated rats. Biochem Anal Biochem 1(8):1–6
Harborne JB (1973) Phenolic compounds. phytochemical methods. Springer, Dordrecht, pp 33–88
Hashem AM, El Sharkawy IN (2009) Hemato-biochemical and immunotoxicological effects of low electromagnetic field and its interaction with lead acetate in mice. Iraqi J Vet Sci 23(3):105–114
Hashish AH, El Missiry MA, Abdelkader HI (2008) Assessment of biological changes of continuous whole-body exposure to static magnetic field and extremely low frequency electromagnetic fields in mice. Ecotoxicol Environ Saf 71(3):895–902
Hassanen MHN (2015) Protective effect of nutmeg and Rosemary on oxidative stress in hypercholesterolemic rats. Int J Nutr Food Sci 4(4):465–476
Hsu WL, Tatukawa Y, Nerishi K, Michiko Y, Cologne J, Fujiwara S (2010) Longitudinal trends of total white blood cell and differential white blood cell counts of atomic bomb survivors. J Radiat Res 51:431–439
Ibrahim M, EL AshryAli ME (2008) The influence of 50 Hz magnetic field on liver function. Rom J Biophys 18(2):113–122
ICNIRP (2010) Int. Commission on non-ionizing radiation protection of workers against ultraviolet radiation. Health Phys. 99 (1), 66-87
Kalpana C, Menon VP (2004) Modulatory effects of curumin on lipid peroxidation and antioxidant status during nicotine induced toxicity. Pol J Pharmacol 56(5):581–586
Karthivashan G, Tangestani FM, Arulselvan P, Abas F, Fakurazi S (2013) Identification of bioactive candidate compounds responsible for oxidative challenge from hydroethanolic extract of Moringa oleifera leaves. J Food Sci 78(9):1368–1375
Kaur S, Mondal P (2014) Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp 1(1):29
Khaki A (2016) Protective effect of Ocimum basilicum on brain cells exposed to oxidative damage by electromagnetic field in rat: ultra structural study by transmission electron microscopy. Crescent J Med Biol Sci 3(1):1–7
Khaki A, Fathiazad F, Nouri M, Khaki AA (2011) Effect of Ocimum basilicum on apoptosis in testis of rats after exposure to electromagnetic field. Afr J Pharm Pharmacol 5(12):1534–1537
Khalafalla MM, Abdellatef E, Dafalla MH, Nassrallah AA, Aboul Enein MK, Lightfoot AD, El Deeb EF, El Shemy AH (2010) Active principle from Moringa oleifera Lam leaves effective against two leukemias and a hepato carcinoma. Afr J Biotechnol 9(49):8467–8471
Khalid S, Shahzad A, Basharat N, Abubakar M, Anwar P (2018) Phytochemical screening and analysis of selected medicinal plants in Gujrat. J Phytochem & Biochem 2(1):108–110
Khamis MI, Aly AA (2017) Preliminary phytochemical screening of different solvent extracts of some medicinal plants. Middle East J Appl Sci 7(2):226–231
Khandelwal KR (2008) Practical Pharmacognosy, 19th edn. Nirali Publication, Pune, p 149–164
Kim IS, Yang MR, Lee OH, Kang SN (2011) Antioxidant activities of hot water extracts from various spices. Int J Mol Sci 12(6):4120–4131
Kula B, Drozdz M (1996) A study of magnetic field effects on fibroblasts cultures, Part 2: The evaluation of effects of static and extremely low frequency (ELF) magnetic fields on free radical processes in fibroblasts cultures. Bioelectrochem Bioenerg 39(1):27–30
Kulkarni AG, Gandhare ZW (2015) Effect of extremely low frequency electric field on liver, kidney, and lipids of Wistar rats. Int J Med Sci Public Health 4(12):1755–1761
Kumar V, Pandey N, Mohan N, Singh RP (2012) Antibacterial and antioxidant activity of different extract of Moringa oleifera leaves – an in-vitro study. Int J Pharm Sci Rev Res 12(1):89–94
Kumar A, Ilavarasan R, Jayachandran T, Decaraman M, Aravindhan P, Padmanaban N, Krishnan MRV (2009) Phytochemical investigation on a tropical plant. Pak J Nutr 8(1):83–85
Labban L, Mustafa UE, Ibrahim YM (2014) The effects of rosemary (Rosmarinus officinalis) leaves powder on glucose level, lipid profile and lipid perodoxation. Int J Clin Med 5(6):297–304
Mansour HH (2013) Protective effect of ginseng against gamma-irradiation-induced oxidative stress and endothelial dysfunction in rats. EXCLI J 12:766–777
Maurya S, Singh D (2010) Quantitative analysis of total phenolic content in Adhatoda vasica nees. Int J Pharmtech Res 2(4):2403–2406
Medini F, Fellah H, Ksouri R, Abdelly C (2014) Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J Taibah Univ Sci 8(3):216–224
Mir MA, Sawhney SS, Jassal MMS (2013) Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker J Phar & Pharmaco 2(1):1–5
Novikov VV, Kuvichkin VV, Fesenko EE (1999) Effect of weak combined low frequency constant and alternative magnetic fields on intrinsic fluorescence of proteins in aqueous solutions. Biofizika 44(2):224–230
Ohkawa H, Ohishi W, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358
Ozguner F, Oktem F, Ayata A, Koyu A, Yilmaz HR (2005) A novel antioxidant agent caffeic acid phenthyle ester prevents long- term mobile phone exposure- induced renal impairment in rat. Mol Cell Biochem 277(1–2):73–80
Pari L, Karthikesan K (2007) Protective role of caffeic acid against alcohol – induced biochemical changes in rats. Fundam Clin Pharmacol 21(4):355–361
Paris R, Moyse H (1969) Precis de matieremedicinale. Ed Masson Paris.
Pawłowska K, Janda K, Jakubczyk K (2020) Properties and use of rosemary (Rosmarinus officinalis L.). Pomeranian J Life Sci 66(3):76–82. https://doi.org/10.21164/pomjlifesci.722
Polterait O (1997) Antioxidants and free-radical scavengers of natural Origin. Curr Org Chem 1(4):415–440
Rahbardar MG, Hosseinzadeh H (2020) Therapeutic effects of rosemary (Rosmarinus officinalis L.) and its active constituents on nervous system disorders. Iran J Basic Med Sci 23(9):1100
Reitman S, Frankel S (1957) A colourimetric method for the determination of serum oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56
Rizk AM (1982) Constituents of plants growing in Qatar. I. a chemical Survey of Sixty plants. Fitoterapia 52:35–44
Salem A, Hafedh A, Rached A, Mohsen S, Khémais BR (2005) Zinc prevents hematological and biochemical alterations induced by static magnetic field in rats. Pharmacol Rep 57(5):616–622
Schermer S (1967) The blood morphology of laboratory animals. Philadelphia, F. A. Davis Co. 3:200
Shanmugavel G, Prabakaran K, George B (2018) Evaluation of phytochemical constituents of Moringa oleifera (lam.) leaves collected from puducherry region, south india. Int J Zool Appl Biosci 3(1):1–8
Sharma S, Sharma A, Shukla S (2017) Effect of electromagnetic radiation on vital organs in rats. Octa J Biosci 5(1):1–4
Snedecor GW, Cochran WG (1986) Statistical methods, 7th edn. Iowa State University Press, Ames, p 90
Sokolovic D, D**djic B, Nikolic J, Bjelakovic G, Pavlovic D, Kocic G (2008) Melatonin reduces oxidative stress induced by chronic exposure of microwave radiation from mobile phones in rat brain. J Radiat Res (tokyo) 49(6):579–586
Thakare SN, Utane SA (2018) Effect of exposure of high frequency electromagnetic field on human health: a review. Int J Adv Res Electron Commun Instrum Eng 7(1):45–49
Treare GE, Evans WC (1985) Pharmacognosy 17th edn, Bahive Tinal, London. 149
Walters MI, Gerarde HW (1970) An ultra-micro method for the determination of conjugated and total bilirubin in serum or plasma. Microchem J 15(2):231–243
WHO (2007) Environmental Health Criteria 238. Extremely low frequency (ELF) fields, World Health Organization, Geneva, p 2007
**ao-Feng P, Gun L (2017) The influences of electromagnetic field irradiated by high voltage transmission lines with 50 Hz on the features of blood in animals. J Tissue Sci Eng 8:194. https://doi.org/10.4172/2157-7552.1000194
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Sallam, K. Tony and Magda, E. Mahmoud: experimental investigation, analysis, data interpretation, writing and editing of original draft.
Dr. Hamadi, A. Ismail, and Fawzy, S. Hatour: conceptuality, writing and editing, experimental investigation of computational study and interfacial behavior.
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Tony, S.K., Ismail, H.A., Hatour, F.S. et al. Hazardous effects of high voltage electromagnetic field on albino rats and protective role of Rosmarinus officinalis. Environ Sci Pollut Res 29, 17932–17942 (2022). https://doi.org/10.1007/s11356-021-17060-x
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DOI: https://doi.org/10.1007/s11356-021-17060-x