Abstract
Gold nanorods (AuNRs) are rod-shaped nanoparticles (NPs) with special optical properties that allow their application in several areas including photothermal therapy, diagnosis, drug and gene delivery, cellular imaging, and biosensors. Their high potential for many applications increases the possibility of release in aquatic environments, which can cause risks to organisms. In this study, we evaluated toxic effects of AuNRs on cladoceran and fish (Ceriodaphnia dubia and Danio rerio) and their recovery after post-exposure periods. The EC50 of 0.03 mg L−1 was found for C. dubia in the acute exposure. There was a significant decrease in the number of neonates produced and in the filtration rate of C. dubia after sub-lethal exposure to AuNRs. The toxic mechanism of these NPs to cladocerans was attributed to increases in the reactive oxygen species (ROS) generation. After 4 h of recovery in clean medium, C. dubia were able to reestablish the filtration rate. Enzymatic biomarkers for D. rerio showed significant increases in the activity of superoxide dismutase, catalase, and lipid peroxidation after sub-lethal exposure to AuNRs. These biomarkers were recovered after 168 h in clean water. These results are pivotal on the comprehension of AuNR toxicity to aquatic organisms and are useful in assessing this novel nanomaterial impacts on aquatic biota.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Centro Nacional de Pesquisa em Energia e Materiais - Brazilian Nanotechnology National Laboratory CNPEM-LNNano (TEM-18453).
Funding
This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (Proc. No. 2014/13493-8) and Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq (Proc. No. 150555/2018-0).
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ASM analyzed the reactive oxygen species generation on C. dubia. FPV analyzed the enzymatic activity of zebrafish and performed the English review of the manuscript. VSM synthesized the AuNRs and performed images of transmission electron microscopy. PMPL characterized the AuNRs including hydrodynamic size and zeta potential in Milli-Q water and exposure media. BPCS cultivated and maintained C. dubia in laboratory and performed experiments of survival and reproduction. BD cultivated zebrafish in laboratory and achieved sub-lethal experiments. JPS performed all analyses on C. dubia and zebrafish, proposed the experimental design, discussed and interpreted the results, and was a major contributor in writing the manuscript. VZ proposed to experimental design, reviewed, and discussed the results. All authors read and approved the final manuscript.
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Souza, J.P., Mansano, A.S., Venturini, F.P. et al. Toxicity of gold nanorods on Ceriodaphnia dubia and Danio rerio after sub-lethal exposure and recovery. Environ Sci Pollut Res 28, 25316–25326 (2021). https://doi.org/10.1007/s11356-021-12423-w
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DOI: https://doi.org/10.1007/s11356-021-12423-w