SpringerLink
Log in

Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

Cr (chromium, with common valence states of III and VI) is one of the common broiler feed additives. Liver injury and metabolic disorders could be caused by Cr(VI) (hexavalent chromium) poisoning in broilers. Oxidative damage and metabolic disorders of organisms caused by heavy metals could be antagonized by nano-Se (nano-selenium). Nano-Se was chosen to study the antagonism of Cr(VI) poisoning in broilers. AMPK (Adenosine 5,-monophosphate-activated protein kinase) is known as a “cell energy regulator” and plays a key regulatory role in carbohydrate and lipid metabolism. AMPK pathway and ACACA/CPT1A two genes were selected to study the prevention and treatment of nano-Se on Cr(VI) poisoning in broilers and its molecular mechanism. For this purpose, 180 1-day-old AA (Arbor Acres) broilers were selected and randomly divided into 6 groups (n = 30) for further testing. After feeding as planned for 35 days, the livers of such broilers were taken for further examination including histopathological examination, differential gene expression analysis, and further validation on both mRNA and protein levels using related techniques like RT-qPCR, western blot, and immunohistochemistry (IHC). The histopathological examination suggested that the liver cells of the Cr(VI) poisoning group were more severely injured than the nano-Se addition group. RT-qPCR results showed that the relative expression of ACACA gene in the Cr(VI) poisoning group was significantly increased (P < 0.05), while the CPT1A gene’s expression was significantly decreased (P < 0.01). Those results were reversed in the nano-Se addition group. Western blot results were consistent with RT-qPCR and both suggested antagonism of nano-Se on Cr(VI). Through morphological and histopathological observation, as well as the measurement of the mRNA and protein expression levels of ACACA and CPT1A genes in AMPK pathway, it was confirmed that nano-Se has certain preventive and protective effects on Cr(VI) poisoning in broiler chickens. Furthermore, the adverse effects of Cr(VI) on carbohydrate and lipid metabolism in broilers can be antagonized by nano-Se through AMPK pathway. A new method and experimental basis were provided to the future study of Cr(VI) poisoning in broilers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  • Alehagen U, Alexander J, Aaseth J (2016) Supplementation with selenium and coenzyme Q10 reduces cardiovascular mortality in elderly with low selenium status. A Secondary Analysis of a Randomised Clinical Trial. Plos One 11:e0157541

    Article  Google Scholar 

  • Al-Quraishy S, Dkhil MA, Moneim AEA (2015) Anti-hyperglycemic activity of selenium nanoparticles in streptozotocin-induced diabetic rats. Int J Nanomedicine 10:6741–6756

    CAS  Google Scholar 

  • Ates B, Orun I, Talas ZS, Durmaz G, Yilmaz I (2008) Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout ( Oncorhynchus mykiss Walbaum, 1792) exposed to Pb 2+ and cu 2+. Fish Physiol Biochem 34:53–59

    Article  CAS  Google Scholar 

  • Chen P, Zhu Y, Wan H, Wang Y, Hao P, Cheng Z, Liu Y, Liu J (2017) Effects of the oral administration of K 2 Cr 2 O 7 and Na 2 SeO 3 on Ca, mg, Mn, Fe, cu, and Zn contents in the heart, liver, spleen, and kidney of chickens. Biol Trace Elem Res 180:285–296

    Article  CAS  Google Scholar 

  • Chinese National feed hygiene standard (2001) NY5037-2001

  • De Flora S, Camoirano A, Bagnasco M, Bennicelli C, Corbett GE, Kerger BD (1997) Estimates of the chromium(VI) reducing capacity in human body compartments as a mechanism for attenuating its potential toxicity and carcinogenicity. Carcinogenesis 18:531

    Article  Google Scholar 

  • Fan W-T, Zhao X-N, Cheng J, Liu Y-H, Liu J-Z (2015) Oxidative stress and hepatocellular injury induced by oral administration of Cr3+ in chicken. J Biochem Mol Toxicol 29:280–287

    Article  CAS  Google Scholar 

  • Hardie DG (2014) AMP-activated protein kinase: maintaining energy homeostasis at the cellular and whole-body levels. Annu Rev Nutr 34:31

    Article  CAS  Google Scholar 

  • Hardie DG (2015) AMP-activated protein kinase: a key regulator of energy balance with many roles in human disease. J Intern Med 276:543–559

  • Hatfield DL, Tsuji PA, Carlson BA, Gladyshev VN (2014) Selenium and selenocysteine: roles in cancer, health, and development. Trends Biochem Sci 39:112–120

    Article  CAS  Google Scholar 

  • Iqbal ZM, Akbar H, Hosseini A, Forteguerri EBR, Osorio JS, Loor JJ (2016) Digital cushion fatty acid composition and lipid metabolism gene network expression in Holstein dairy cows fed a high-energy diet. PLoS One 11:e0159536

    Article  Google Scholar 

  • Jafari Dehkordi A, Mohebbi AN, Aslani MR, Ghoreyshi SM (2017) Evaluation of nanoselenium (Nano-Se) effect on hematological and serum biochemical parameters of rat in experimentally lead poisoning. Hum Exp Toxicol 36(4):421–427

  • Jaroslav R, Sindberg CD, Moesgaard S, Josef M, Jaroslav F, Luděk M, Katarína R (2013) Effect of chromium-enriched yeast on fasting plasma glucose, glycated haemoglobin and serum lipid levels in patients with type 2 diabetes mellitus treated with insulin. Biol Trace Elem Res 155:1–4

    Article  Google Scholar 

  • Jeyasingh J, Philip L (2005) Bioremediation of chromium contaminated soil: optimization of operating parameters under laboratory conditions. J Hazard Mater 118:113–120

    Article  CAS  Google Scholar 

  • Khorsandi K, Rabbani-Chadegani A (2013) Studies on the genotoxic effect of chromium oxide (Cr VI): interaction with deoxyribonucleic acid in solution. Mutat Res 750:105–110

    Article  CAS  Google Scholar 

  • Król E, Michalak S, Wójciak RW, Bogdański P (2012) Effects of combined dietary chromium(III) propionate complex and thiamine supplementation on insulin sensitivity, blood biochemical indices, and mineral levels in high-fructose-fed rats. Biol Trace Elem Res 150:350–359

    Article  Google Scholar 

  • Lau FC, Bagchi M, Sen CK, Bagchi D (2008) Nutrigenomic basis of beneficial effects of chromium(III) on obesity and diabetes. Mol Cell Biochem 317:1–10

    Article  CAS  Google Scholar 

  • Lipinski B (2017) Sodium selenite as an anticancer agent. Anti Cancer Agents Med Chem 17:658–661

    Article  CAS  Google Scholar 

  • Liu X, Rehman MU, Mehmood K, Huang S, Tian X, Wu X, Zhou D (2018) Ameliorative effects of nano-elemental selenium against hexavalent chromium-induced apoptosis in broiler liver. Environ Sci Pollut Res Int 25:15609

    Article  CAS  Google Scholar 

  • Loeschner K, Hadrup N, Hansen M, Pereira SA, Gammelgaard B, Møller LH, Mortensen A, Lam HR, Larsen EH (2014) Absorption, distribution, metabolism and excretion of selenium following oral administration of elemental selenium nanoparticles or selenite in rats. Metallomics 6:330–337

    Article  CAS  Google Scholar 

  • Malhotra S, Welling MN, Mantri SB, Desai K (2015) In vitro and in vivo antioxidant, cytotoxic, and anti-chronic inflammatory arthritic effect of selenium nanoparticles. J Biomed Mater Res B Appl Biomater 104:993–1003

    Article  Google Scholar 

  • Munehiro Y (2012) Is chromium an essential trace element in human nutrition? Nihon Eiseigaku Zasshi 67:485

    Article  Google Scholar 

  • Nejla S, Mediha S, Ibtissem BA, Tahia B, Najiba Z (2010) Protective effects of selenium (se) on chromium (VI) induced nephrotoxicity in adult rats. Ecotoxicol Environ Saf 73:671–678

    Article  Google Scholar 

  • Passerieux E, Hayot M, Jaussent A, Carnac G, Gouzi F, Pillard F, Picot MC, Böcker K, Hugon G, Pincemail J (2015) Effects of vitamin C, vitamin E, zinc gluconate, and selenomethionine supplementation on muscle function and oxidative stress biomarkers in patients with facioscapulohumeral dystrophy: a double-blind randomized controlled clinical trial. Free Radic Biol Med 81:158–169

    Article  CAS  Google Scholar 

  • Pati A, Chaudhary R, Subramani S (2014) A review on management of chrome-tanned leather shavings: a holistic paradigm to combat the environmental issues. Environ Sci Pollut Res Int 21:11266–11282

    Article  CAS  Google Scholar 

  • Ray RR (2016) Adverse hematological effects of hexavalent chromium: an overview. Interdiscip Toxicol 9:55–65

    Article  CAS  Google Scholar 

  • Saeed AA, Sandhu MA, Khilji MS, Yousaf MS, Rehman HU, Tanvir ZI, Ahmad T (2017) Effects of dietary chromium supplementation on muscle and bone mineral interaction in broiler chicken. J Trace Elem Med Biol 42:25

    Article  CAS  Google Scholar 

  • Saez G, Davail S, Gentès G, Hocquette JF, Jourdan T, Degrace P, Baéza E (2009) Gene expression and protein content in relation to intramuscular fat content in Muscovy and Pekin ducks. Poult Sci 88:2382–2391

    Article  CAS  Google Scholar 

  • Sahin N, Hayirli A, Orhan C, Tuzcu M, Akdemir F, Komorowski JR, Sahin K (2017) Effects of the supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress. Poult Sci:96

  • Siddiqui MA, Alhadlaq HA, Javed A, Al-Khedhairy AA, Javed M, Maqusood A (2013) Copper oxide nanoparticles induced mitochondria mediated apoptosis in human hepatocarcinoma cells. PLoS One 8:e69534

    Article  CAS  Google Scholar 

  • State G, Popescu IV, Radulescu C, Macris C, Stihi C, Gheboianu A, Dulama I, Niţescu O (2012) Comparative studies of metal air pollution by atomic spectrometry techniques and biomonitoring with moss and lichens. Bull Environ Contam Toxicol 89:580–586

    Article  CAS  Google Scholar 

  • Suzuki K, Nagara H (1981) Brindled mottled mouse: morphological changes of brain and visceral organs in hemizygous males following copper supplementation. Acta Neuropathol 55:251

    Article  CAS  Google Scholar 

  • Tian X, Zhang H, Zhao Y, Mehmood K, Wu X, Chang Z, Luo M, Liu X, Ijaz M, Javed MT (2018) Transcriptome analysis reveals the molecular mechanism of hepatic metabolism disorder caused by chromium poisoning in chickens. Environ Sci Pollut Res Int 25:1–11

    Google Scholar 

  • Wan H, Zhu Y, Chen P, Wang Y, Hao P, Cheng Z, Liu Y, Liu J (2017) Effect of various selenium doses on chromium(IV)-induced nephrotoxicity in a male chicken model. Chemosphere 174:306

    Article  CAS  Google Scholar 

  • Wang Y, Tang JW, Ma WQ, Feng J, Feng J (2010) Dietary zinc glycine chelate on growth performance, tissue mineral concentrations, and serum enzyme activity in weanling piglets. Biol Trace Elem Res 133:325–334

    Article  CAS  Google Scholar 

  • Whanger PD (2002) Selenocompounds in plants and animals and their biological significance. J Am Coll Nutr 21:223–232

    Article  CAS  Google Scholar 

  • Xu X, Yekeen TA, Liu J, Zhuang B, Li W, Huo X (2015) Chromium exposure among children from an electronic waste recycling town of China. Environ Sci Pollut Res 22:1778–1785

    Article  CAS  Google Scholar 

  • Zhao Y, Zhang H, Wu X et al (2019) Metabonomic analysis of the hepatic injury suffer from hexavalent chromium poisoning in broilers. Environ Sci Pollut Res 26:18181

    Article  CAS  Google Scholar 

  • Zheng-Jiang Z, Schultz AW, Junhua W, Johnson CH, Yannone SM, Patti GJ, Gary S (2013) Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database. Nat Protoc 8:451–460

    Article  Google Scholar 

Download references

Funding

This study was supported by the National Key R&D Program of China (2016YFD0501208) and National Natural Science Foundation of China (No. 31972748).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Veterinary clinical medicine laboratory, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, 430070, China

    Tian-guang Zhang, Ya-li Zhao, Lei Li & Dong-hai Zhou

Authors
  1. Tian-guang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ya-li Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dong-hai Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Z.T., Z.Y., and Z.D. were responsible for study conception and design; L.L. was involved in the drafting of the manuscript.

Corresponding author

Correspondence to Dong-hai Zhou.

Ethics declarations

Conflict of interest

The authors declared that they have no conflicts of interest.

Additional information

Responsible Editor: Mohamed M. Abdel-Daim

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Tg., Zhao, Yl., Li, L. et al. Antagonistic effects of nano-selenium on broilers hepatic injury induced by Cr(VI) poisoning in AMPK pathway. Environ Sci Pollut Res 27, 41585–41595 (2020). https://doi.org/10.1007/s11356-020-08501-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-020-08501-0

Keywords

Search

Navigation