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Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

  • Multi-Stressors in Freshwater and Transitional Environments: from Legacy Pollutants to Emerging Ones
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Abstract

The European sturgeon, Acipenser sturio, is a highly endangered species that almost disappeared in the last decades. Thanks to yearly restocking of the population, this species is still found in the Gironde estuary (France), where juveniles grow during several years before leaving to the ocean. The aims of this study were to evaluate the pressure exerted on these fish by known organic and inorganic contaminants during their stay at the Gironde estuary, and to get information on the fish’s health in this context. Monthly captures over the year 2014 provided 87 fish from the cohorts 2012 and 2013 mainly, and from cohorts 2008, 2009, and 2011, all fish born in hatchery. We report the very first analyses of contaminant levels and of biological markers measured in the blood of these fish. Low inorganic contamination was found, composed of seven metals mainly Zn (< 5 μg mL−1), Fe (< 1.5 μg mL−1), Cu (< 0.8 μg mL−1), Se (< 0.8 μg mL−1), As (< 0.25 μg mL−1), Co (< 0.14 μg mL−1), and Mn (< 0.03 μg mL−1). Concerning persistent organic contaminants, the sum of seven PCBs varied from 1 to 10 ng g−1 plasma, that of eight OCPs from 0.1 to 1 ng g−1, and that of eight PBDEs from 10 to 100 pg g−1. Higher levels of contaminants were measured during spring as compared to summer. The sex steroid hormone plasma levels (estradiol, testosterone, and 11-ketotestosterone) were quite low, which was predictable for juveniles. The transcription of reproduction-involved genes (EstR, AR, LHR, sox9) in blood cells was demonstrated for the first time. Some of them were correlated with organic contaminant levels PCBs and OCPs. Other gene transcriptions (sodCu and bax) were correlated with PCBs and OCPs. However, the DNA damage level measured here as comet tail DNA and micronuclei ratio in red blood cells were in the very low range of the values commonly obtained in fish from pristine areas. The data presented here can serve as a reference base for future monitoring of this population of sturgeons.

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Acknowledgments

We wish to thank J.F. Bigot and B. Ballion (IRSTEA) for managing trawl fishing operation as well as J. Kordek (IRSTEA), F. Le Bihanic-Michel (EPOC), and M-A. Cornet (EPOC) who occasionally participate to the field sampling. We wish to thank Hélène Bouteiller and Elea Barjavel for taking part to qPCR measurements.

Funding

This study was funded by the Région Aquitaine, IRSTEA, and the French National Research Agency (SturTOP research project no. ANR-13-CESA-0018-01). It was carried out as part of the Cluster of Excellence COTE. Sampling campaigns were funded within the frame of the French National Action Plan in favor of A. sturio restoration by Adour-Garonne water agency, Aquitaine region, and Gironde general council in 2014.

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Author notes

  1. Marie-Laure Acolas and Blandine Davail contributed equally to this work.

Authors and Affiliations

  1. Irstea, EABX, Aquatic Ecosystems and Global Changes, 50 avenue de Verdun, F-33612, Gazinet Cestas, France

    Marie-Laure Acolas, Maud Pierre, Romaric Le Barh & Eric Rochard

  2. University of Bordeaux, UMR 5805 EPOC, Aquatic Ecotoxicology, B2, allée Geoffroy Saint-Hilaire, F-33405, Talence, France

    Blandine Davail, Christelle Clérandeau, Bénédicte Morin & Jérôme Cachot

  3. University of Bordeaux, UMR 5805 EPOC, Aquatic Ecotoxicology, Place du Dr B. Peyneau, F-33120, Arcachon, France

    Patrice Gonzalez, Pierre-Yves Gourves, Guillemine Daffe & Magalie Baudrimont

  4. EcoLab, Université de Toulouse, CNRS, INPT, UPS, Avenue de l’Agrobiopole, F-31326, Caxtanet-Tolosan Cedex, France

    Séverine Jean & Annie Perrault

  5. University of Bordeaux, UMR 5805 EPOC, LPTC, 351 crs de la Libération, F-33405, Talence, France

    Pierre Labadie, Mathilde Lauzent, Laurent Peluhet, Karyn Le Menach & Hélène Budzinski

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  1. Marie-Laure Acolas
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Contributions

ML.A., B.D., B.M., P.G., H.B., S.J., E.R., and J.C. conceived and designed the experiments. All authors contributed to the experiments. ML.A, B.D., S.J., C.C, M.L., and R.LB. did the samplings on fish onboard. B.D., P-Y.G., G.D., A.P., M.L, L.P., K.LM., and C.C. made the assays. ML.A, B.D., M.P., M.B, and C.C. managed with the statistical analyses of the data. ML.A and B.D. wrote the paper. E.R. and J.C. supervised the SturTOP research project.

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Correspondence to Blandine Davail.

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Acolas, ML., Davail, B., Gonzalez, P. et al. Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon. Environ Sci Pollut Res 27, 3726–3745 (2020). https://doi.org/10.1007/s11356-019-05139-5

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