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Proteomics and 1H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains

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Abstract

Vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. Vibrio vulnificus is a pathogenic bacterium that infects people and livestock. It is usually found in offshore waters or within fish and shellfish. This study presents a comparative proteomic analysis of the outer membrane protein (OMP) changes in V. vulnificus proteins after stimulation with sewage from sewage drains. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 32 protein spots with significant differences in abundance were identified and characterized. These identified proteins were found to be involved in various functional categories, including catalysis, transport, membrane proteins progresses, receptor activity, energy metabolism, cytokine activity, and protein metabolism. The mRNA expression levels of 12 differential proteins were further assessed by qRT-PCR. Seven genes including carboxypeptidase, hemoglobin receptor, succinate dehydrogenase iron-sulfur subunit, ATP synthase subunit alpha, thioredoxin, succinyl-CoA synthetase subunit, and alanine dehydrogenase were downregulated upon stimulation, whereas the protein expression levels HupA receptor, type I secretion outer membrane protein, glutamine synthetase, superoxide dismutase, OmpU, and VuuA were upregulated. 1H NMR spectra showed 18 dysregulated metabolites from V. vulnificus after the sewage stimulation and the pathogenicity was enhanced after that.

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Acknowledgements

This work was supported by the Public Science and Technology Research Funds ocean projects (201105007, 201005016), the Regional Demonstration Project of Marine Economic Innovation and Development (2014, 2016), the Scientific Research Foundation of the Graduate School of Ningbo University, and K.C. Wong Magna Fund in Ningbo University. We thank the American Journal Experts (AJE) for English language editing. This manuscript was edited for English language by the American Journal Experts (AJE).

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  1. School of Marine Science, Ningbo University, Ningbo, China

    Chundan Zhang, Zhonghua Wang, Dijun Zhang, Jun Zhou, Chenyang Lu, **urong Su & Dewen Ding

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  1. Chundan Zhang
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  2. Zhonghua Wang
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  4. Jun Zhou
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  6. **urong Su
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Correspondence to **urong Su or Dewen Ding.

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Zhang, C., Wang, Z., Zhang, D. et al. Proteomics and 1H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains. Environ Sci Pollut Res 24, 23704–23713 (2017). https://doi.org/10.1007/s11356-017-0007-1

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