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DNA fingerprinting of oil camellia cultivars with SSR markers

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Abstract

Oil camellia (Camellia oleifera Abel.) is a widely cultivated economically important plant. In China, more than 100 elite cultivars have been authorized for commercial plantations, representing the developmental base for the oil camellia industry. Since oil camellia seedlings are frequently traded and introduced to new places, there is an active demand to develop a rapid and reliable identification toolkit to protect and ensure the effective use of these resources. In this study, ten highly polymorphic simple sequence repeat (SSR) primer pairs were screened from the SSR primer database of oil camellia, and DNA fingerprints of 128 elite oil camellia cultivars were constructed with these primer pairs. By analyzing the fingerprinting profiles, all of these cultivars were found to have unique DNA fingerprints, except ‘Changlin 40’ and ‘Fuyang 40’, which shared a complete similarity across all of the fingerprints generated by these ten SSR primer pairs, indicating that they were either synonyms or vegetative mutants. Synonyms are a major source of confusion in protecting and managing new varieties of plants. Although the synonymy of these two cultivars needs to be further confirmed, our data indicated a high possibility of synonyms among the authorized oil camellia cultivars. This study provides a desirable genetic toolkit for the identification and management of the elite oil camellia cultivars, and also for the intellectual property right protection of breeders.

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Acknowledgments

We thank Prof. Shuxian Li and Jiseng Shi at the Southern Forest Tree Seeds Inspection Center of China for their help in collecting the elite oil camellia cultivars. This work was funded by the 948 Project (2014-4-13), the Natural Science Foundation of China and Jiangsu Province (31400564, 31270711, and BK20130968). It was also supported by Jiangsu Planned Projects for Postdoctoral Research Funds (1402011B), the Innovative Research Team Program in Jiangsu Province and the Educational Department of China, and the Priority Academic Program Development (PAPD) Program at Nan**g Forestry University.

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    Authors and Affiliations

    1. The Southern Modern Forestry Collaborative Innovation Center, Nan**g Forestry University, Nan**g, 210037, China

      Yingnan Chen, **aogang Dai, **g Hou, Hongwei Guan, Yuxiao Wang & Tongming Yin

    2. Department of Plant Science and Landscape architecture, University of Connecticut, Storrs, CT, 06269, USA

      Yi Li

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    1. Yingnan Chen
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    Correspondence to Tongming Yin.

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    Communicated by M. Wirthensohn

    Yingnan Chen and **aogang Dai contributed equally to this work.

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    Table S1

    Detailed information of the examined oil camellia cultivars.(XLS 21 kb)

    Table S2

    A summary of the allele sizes amplified in each cultivar by each of the SSR primers.(XLS 32 kb)

    Table S3

    Genotypes detected by each SSR primer and the included cultivars with the same genotype at each SSR locus.(XLS 42 kb)

    Table S4

    The frequency of each cultivar’s genotype at each of the SSR loci.(XLS 27 kb)

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    Chen, Y., Dai, X., Hou, J. et al. DNA fingerprinting of oil camellia cultivars with SSR markers. Tree Genetics & Genomes 12, 7 (2016). https://doi.org/10.1007/s11295-015-0966-7

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    • DOI: https://doi.org/10.1007/s11295-015-0966-7

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