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Screening and identification of specific cluster miRNAs in N2a cells infected by H7N9 virus

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Abstract

This study aims to screen and identify specific cluster miRNAs of H7N9 virus-infected N2a cells and explore the possible pathogenesis of these miRNAs. The N2a cells are infected with H7N9 and H1N1 influenza viruses, and the cells are collected at 12, 24 and 48 h to extract total RNA. To sequence miRNAs and identify different virus-specific miRNAs, high-throughput sequencing technology is used. Fifteen H7N9 virus-specific cluster miRNAs are screened, and eight of them are included in the miRBase database. These cluster-specific miRNAs regulate many signaling pathways, such as the PI3K-Akt signaling pathway, the RAS signaling pathway, the cAMP signaling pathway, actin cytoskeleton regulation and cancer-related genes. The study provides a scientific basis for the pathogenesis of H7N9 avian influenza, which is regulated by miRNAs.

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Data availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

National Natural Science Foundation of China (No.81871631), Title: Study on the Mechanism of PAFAH-PAF Unbalance on H7N9 Influenza Virus Encephalopathy; Shenzhen Science and Technology Plan Project (JCYJ20180307102005105).

Author information

Authors and Affiliations

  1. **an University, College of Pharmacy, Guangzhou, 510632, China

    Yitong Yin & Qing Zheng

  2. Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China

    Ying Sun, Hui Liu, Weihua Wu, **n Wang & Shisong Fang

  3. National Sun yat-sen University, Public Health Hospital (Shenzhen), Shenzhen, 518107, China

    Zengzhao Qiu, Yuxuan Lei & Yuelong Shu

  4. Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College CN, Bei**g, 102206, China

    Yuelong Shu

  5. Guangdong Pharmaceutical University, Public Health College, Guangzhou, 510006, China

    Jia Huang

Authors
  1. Yitong Yin
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  2. Zengzhao Qiu
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  3. Yuxuan Lei
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  4. Jia Huang
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  5. Ying Sun
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  6. Hui Liu
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  7. Weihua Wu
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  8. **n Wang
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  9. Yuelong Shu
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  10. Qing Zheng
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  11. Shisong Fang
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Contributions

YY and JH formulated the idea of the article, performed the research, and analyzed the data. YY wrote the manuscript. ZQ, YL, YS, HL, WW and WX revised the data and improved manuscript writing. YS, QZ, and SF were responsible for the planning and coordination of the research activity and the acquisition of the financial support for the project leading to this publication. All authors reviewed the manuscript and approved the final version of the manuscript.

Corresponding authors

Correspondence to Qing Zheng or Shisong Fang.

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The authors declare no conflict of interest.

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Edited by Zhen Fu.

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Yin, Y., Qiu, Z., Lei, Y. et al. Screening and identification of specific cluster miRNAs in N2a cells infected by H7N9 virus. Virus Genes 59, 716–722 (2023). https://doi.org/10.1007/s11262-023-01996-y

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  • DOI: https://doi.org/10.1007/s11262-023-01996-y

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