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Molecular epidemiology of PRRSV from China’s Guangxi Province between 2007 and 2009

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Abstract

Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases affecting livestock. This study used gene sequence analysis of ORF5 and Nsp2 to determine the molecular epidemiology of PRRSV in different parts of the Guangxi province of China. These genes were selected due to their extensive variation within the genome. Out of 189 samples from animals suspected to have PRRS, 145 were PRRSV RNA positive. ORF5 and Nsp2 gene sequence analysis of 31 of these samples showed that all of the Guangxi isolates were of type 2. A phylogenetic tree analysis based on ORF5 showed that the Guangxi isolates were divided into two groups. Most of these were closely related to highly pathogenic strains, showing a 30 amino acid deletion at positions 481 and 533–561 of Nsp2, but an additional unique isolate (GXNN06) possessed a further four amino acid deletion at positions 485–488 of Nsp2.

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Acknowledgments

The study was supported by the Key Project for Scientific-Technology Research & Development of the Guangxi Zhuang Autonomous Region (Guikegong 0322004-2C).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

    **g-**g Liang, **ao-ning Li & Ting Rong Luo

  2. College of Animal Sciences and Veterinary Medicine, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

    Hong-Yun Zhang, **g-**g Liang, **an-Ming Meng, Hui Li, Jian Yang, Li-Juan Su, Hong-Pu Zhang, Lin-Juan **e, **ao-**a He, Yan-Sheng Li, Shan Yin, **ao-Quan Li, **ao-ning Li & Ting Rong Luo

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Zhang, HY., Liang, JJ., Meng, XM. et al. Molecular epidemiology of PRRSV from China’s Guangxi Province between 2007 and 2009. Virus Genes 46, 71–80 (2013). https://doi.org/10.1007/s11262-012-0824-8

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  • DOI: https://doi.org/10.1007/s11262-012-0824-8

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