Abstract
As is the case with other veterinary antibiotics, florfenicol (FFC) faces certain limitations, such as low solubility in water, or the fact that it is reported to interfere with the immune response after some immunoprofilactic actions in livestock. Aiming to improve its efficacy and overall performance, FFC was loaded into a polymeric nanobased delivery system by succesfully using the emulsion-evaporation technique. The poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with FFC were characterized in terms of size (101 ± 0.52 nm), zeta potential (26.80 ± 1.30 mV) and poly-dispersity index (0.061 ± 0.019). The achieved loading was 2.24 μg FFC/mg of NPs, with an entrapment efficiency of 7.9%. The antimicrobial effect, the anti-biofilm formation and the cytotoxicity properties of the NPs were evaluated. The results indicated a MIC decreased by ~97.13% for S. aureus, 99.33% for E.coli and 64.1% for P. aeruginosa when compared to free FFC. The minimum inhibitory concentration (MIC) obtained indicated the potential for using a significantly lower dose of florfenicol. The delivery system produced biofilm inhibition while showing no cytotoxic effects when tested on porcine primary fibroblasts and horse mesenchymal stem cells. These findings suggest that florfenicol can be improved and formulations optimized for use in veterinary medicine through its incorporation into a nanobased delivery system designed to release in a controlled manner over time.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgments
This work was supported by a grant of the Ministry of Research, Innovation and Digitization, CNCS -UEFISCDI, project number PN-III-P1-1.1-PD-2021-0033, within PNCD III, as well as by the USDA National Institute of Food and Agriculture, AFRI projects # 2017-07878 and # 2018-07406, and the USDA-NIFA Hatch Project #1008750.
Funding
This work was supported by a grant of the Ministry of Research, Innovation and Digitization, CNCS -UEFISCDI, project number PN-III-P1-1.1-PD-2021-0033, within PNCD III, as well as USDA National Institute of Food and Agriculture, AFRI projects # 2017-07878 and # 2018-07406, and the USDA-NIFA Hatch Project #1008750.
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Conceptualization: Constantin Cerbu and Cristina M. Sabliov; Methodology: Emilia Trif, Constantin Cerbu, Carlos E. Astete, Sumit Libi and Emoke Pall; Formal analysis and investigation: Emilia Trif, Constantin Cerbu, Carlos E. Astete, Sumit Libi, Emoke Pall, Septimiu Tripon, Diana Olah, Adrian Valentin Potarniche, Writing - original draft preparation: Emilia Trif; Writing - review and editing: Constantin Cerbu, Cristina M. Sabliov, Carlos E. Astete, Sumit Libi, Lucjan Witkowski and Marina Spinu; Funding acquisition: Constantin Cerbu and Cristina M. Sabliov; Resources: Constantin Cerbu, Cristina M Sabliov, and Marina Spinu; Supervision: Gheorghe Florinel Brudasca and Cristina M. Sabliov.
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Trif, E., Cerbu, C., Astete, C.E. et al. Delivery of florfenicol in veterinary medicine through a PLGA-based nanodelivery system: improving its performance and overcoming some of its limitations. Vet Res Commun 48, 259–269 (2024). https://doi.org/10.1007/s11259-023-10205-y
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DOI: https://doi.org/10.1007/s11259-023-10205-y