Abstract
The occurence and spread of animal-derived methicillin resistant staphylococci (MRS) worldwide is a current problem, especially due to their increasing incidence in food animals and their products, with possible contamination of food consumers and handlers. Staphylococci isolated from animals (n = 123) were identified with MALDI-TOF mass spectrometry and screened for methicillin/oxacillin/cefoxitin resistance (MR) using the disk diffusion method. Twenty-three phenotypically MRS strains were analysed using PBP2' Latex Agglutination Test Kit to confirm the phenotypic MR and PCR was performed for mecA gene detection; mecA gene positive strains were furtherly confirmed by means of sequencing. The susceptibility of MRS to 11 partially-purified enterocins (Ent) produced by E. faecium, E. durans and E. mundtii strains of animal, feed/food and environmental origin was checked using agar spot tests. Out of 23 MRS, PBP testing confirmed MR in 17 strains. Three Staphylococcus epidermidis and one S. vitulinus were mecA positive. The majority of MRS, including two mecA gene-positive strains S. epidermidis R44/1 and P3/Tr2a, were susceptible to the tested enterocins, mainly to Ent7420, EntA(P)/EK13, Ent412, Ent55 and Ent9296 (in the range 100 – 12,800 AU/mL). The most susceptible strains appeared to be the mecA gene-positive S. epidermidis SE R44/1 and SE P3/Tr2a strains, inhibited by eight enterocins out of 11 tested (100–200 AU/mL). Only four strains (including mecA gene positive S. epidermidis SE P3/Tr1 and S. vitulinus SV K12PL/1) were resistant to the tested antimicrobial substances. These results indicate that the enterocins used offer a promising option for prevention and treatment of bacterial infection caused by MRS in animals.
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Abbreviations
- MDRS:
-
Multidrug-resistant staphylococci
- MRS:
-
Methicillin-resistant staphylococci
- MRSA:
-
Methicillin-resistant Staphylococcus aureus
- MR-CoNS:
-
Methicillin-resistant coagulase-negative staphylococci
- VRSA:
-
Vancomycin-resistant Staphylococcus aureus
- VRS:
-
Vancomycin-resistant staphylococci
- CoPS:
-
Coagulase positive staphylococci
- CCM:
-
Czech Culture Collection of Microorganisms
- Ent:
-
Enterocin
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Acknowledgements
The authors would like to thank Mrs. M. Bodnárová and D. Melišová for excellent assistance in the laboratory. We thanks Mr. A. Billingham for his English language editing.
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This study was financially supported by the project VEGA 2/0005/21 (Slovak Scientific Agency).
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M. Pogány Simonová: Conceptualization, Data curation, Funding acquisition, Investigation, Project administration, Resources, Validation, Methodology, Writing—original draft preparation, Writing—review and editing; M. Maďar: Methodology; A. Lauková: Conceptualization.
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All care and experimental procedures involving animals followed the guidelines stated in the Guide for the Care and Use of Laboratory Animals approved by the Slovakian State Veterinary and Food Administration and the Ethical Committees of both institutions (permission code: SK CH 17016 and SK U 18016).
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Simonová, M.P., Maďar, M. & Lauková, A. Effect of enterocins against methicillin-resistant animal-derived staphylococci. Vet Res Commun 45, 467–473 (2021). https://doi.org/10.1007/s11259-021-09841-z
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DOI: https://doi.org/10.1007/s11259-021-09841-z