Abstract
Tai** chicken is indigenous chickens (Gallus gallus domesticus), which was one of China’s excellent poultry species. As the problems caused by the overuse of antibiotics become more and more concern, people begin to look for ways to replace them. Among them, probiotics and their preparations are the research hotspot to replace antibiotics. Probiotics can promote the absorption of nutrients, improve the ratio of meat to meat, resist and prevent diseases, and improve the intestinal tissue morphology. Here, we performed transcriptome profiling of Tai** chicken ileum which was given probiotics by using the Hiseq™ 2500 sequencing platform. A total of 18 genes were differentially expressed in the ileum under control group and probiotics group. Thirteen genes were upregulated with a range of fold change from 1.02 to 8.61, and 5 were downregulated with a range of fold change from − 1.06 to − 2.29. Ten of the differently expressed genes were further validated by qRT-PCR. In addition, Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that these differentially expressed genes were mainly enriched to bile secretion, alpha-linolenic acid metabolism, linoleic acid metabolism, ether lipid metabolism, and arachidonic acid metabolism. This study will help us to understand the role of probiotics in indigenous chicken production and provide theoretical basis for the genetic development of indigenous chickens.
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This work was supported by the Science and Technology Support Plan Project of Tianshui (No. 2018-FZJHK-7879).
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All the methods used in this study were carried out according to the guiding principles of the Chinese Legislation on the Use and Care of Laboratory Animals. Protocol was approved by the Institutional Ethic Committee of Tianshui normal university. (TSNU).
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Luo, L., Wang, Q. & Ma, F. RNA-Seq transcriptome analysis of ileum in Tai** chicken supplemented with the dietary probiotic. Trop Anim Health Prod 53, 131 (2021). https://doi.org/10.1007/s11250-021-02566-w
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DOI: https://doi.org/10.1007/s11250-021-02566-w