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In vitro studies of epicotyl and embryo culture in cultivated, wild and inter-specific hybrids of okra (Abelmoschus spp.)

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Abstract

The present study aimed to determine the optimal concentration of growth regulators for regenerating Abelmoschus species through epicotyl and embryo culture. Five popular okra genotypes (four varieties, one inbred), two wild species and interspecific hybrids from crossing var. Pusa Sawani with wild relatives were used to develop in vitro regeneration protocol. SSR primers confirmed the hybridity of interspecific crosses. A total of 64 different combinations of indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and kinetin, referred to as MSOM1 to MSOM64 were used to test regeneration efficiency. Among these combinations, MS medium supplemented with NAA and IBA each with concentration of 0.5-1 mg/L and kinetin @ 1- 1.5 mg/L proved to be effective for regeneration using epicotyl and embryo explants in cultivated okra genotypes, wild species and interspecific hybrids. Regeneration efficiency in cultivated genotypes through epicotyl culture ranged from 40 to 65%. However, for both cultivated genotypes and wild species regeneration efficiency ranged from 25 to 65% by embryo culture. On an average, interspecific hybrids achieved regeneration efficiency of 35% to 75% through embryo culture. The present study indicated that the success of apical shoot culture and embryo culture in okra is influenced by the genotype, type of growth hormones and their concentrations.

Key message

In this paper we have optimized regeneration protocol using epicotyl and embryo explants in Abelmoschus spp. for advancement of pre-breeding materials as well as utilization in genetic engineering and genome editing.

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Data availability

All relevant data are within the manuscript and the supplemental materials.

Abbreviations

IBA:

Indole-3-butyric acid

NAA:

Naphthalene acetic acid

MSOM:

MS okra medium

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Acknowledgements

The First author is grateful to the Indian Council of Agriculture Research New Delhi, for providing Senior Research Fellowship, during PhD programme. Authors acknowledge Director, Indian Agricultural Research Institute, New Delhi for providing facility to conduct experiment. We acknowledge Dr. Krishan Kumar, Scientist, ICAR—Indian Institute of Maize Research (IIMR) for critically reading the manuscript and providing valuable inputs. We also acknowledge Mr. Prakash Kumar, Scientist, ICAR- Indian Agricultural statistics Research Institute, New Delhi for performing GLM software analysis.

Funding

First author received Senior Research Fellowship from Indian Council of Agriculture Research, New Delhi. Funding received from Divisional budget, Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

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Authors and Affiliations

  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Bhargav S. Kiran, Ramesh Kumar Yadav, Suman Lata, Brij Bihari Sharma & Bhoopal Singh Tomar

  2. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 11002, India

    A. Talukdar

  3. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 11002, India

    Amalendu Ghosh

  4. ICAR- National Institute for Plant Biotechnology, New Delhi, 11002, India

    Navin Chandra Gupta

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  1. Bhargav S. Kiran
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Contributions

Conceived and designed the experiments: RKY, SL; Performed the experiments: SBK; Analyzed the data: SBK, RKY, BBS; Contributed reagents/materials/analysis tools: RKY, BST, SL, AT; Wrote the paper and edited the manuscript: SBK, SL, RKY, BBS, NCG, AG.

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Correspondence to Ramesh Kumar Yadav or Suman Lata.

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Communicated by Ranjith Pathirana

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Kiran, B.S., Yadav, R.K., Lata, S. et al. In vitro studies of epicotyl and embryo culture in cultivated, wild and inter-specific hybrids of okra (Abelmoschus spp.). Plant Cell Tiss Organ Cult 156, 80 (2024). https://doi.org/10.1007/s11240-024-02699-4

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