Abstract
Triploid Atractylodes chinensis (DC.) Koidz. is a valuable genetic resource for medicinal plant breeding, and there is a demand to develop an efficient in vitro regeneration protocol to resolve triploid sterility in production. In this study, the effects of sterilization, bud type, 6-benzyladenine (6-BA) combined with 1-naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA) and transplanting materials on shoot regeneration, rooting and growth were explored. The genetic stability of regenerated plants was verified using flow cytometry (FCM) and simple sequence repetition (SSR) molecular markers. When axillary buds were sterilized with sodium hypochlorite for 30 min and cultured on Murashige and Skoog (MS) + 1.5 mg·L-1 6-BA + 0.4 mg·L-1 NAA medium, the regeneration rate was 61.33%. Strong roots occurred on 1/2 MS medium combined with 3% sucrose and 0.6 mg·L-1 IBA. Rooting plantlets transplanted into a mixture of peat soil: perlite (1:1, v:v), the survival rate up to 90.00%. Histological analysis revealed that shoot meristematic cells derived from cambium cells developed into the apical meristem and formed shoots. FCM analysis determined that the ploidy levels of 20 random regenerated plants were coincident with explants, the number and size of bands obtained by 6 pairs of SSR polymorphic primers were consistent. The results of this study show that our in vitro adventitious regeneration and plantlet transplantation protocol of triploid A. chinensis was efficient and produced strong genetic stability.
Key message
In this study, an efficient and genetically stable adventitious regeneration of triploid A. chinensis was established for the first time.
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Abbreviations
- AR:
-
Atractylodis Rhizoma
- TCM:
-
Traditional Chinese medicine
- 6-BA:
-
6-Benzyladenine
- CD:
-
Chlorine dioxide
- FCM:
-
Flow cytometry
- MC:
-
Mercuric chloride
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog
- SH:
-
Sodium hypochlorite
- NAA:
-
1-Naphthaleneacetic acid
- PGRs:
-
Plant growth regulators
- SSR:
-
Simple sequence repetition
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This work was supported by the Key Research and Development Project Fund of Jilin Province (20200404005YY).
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Jia, W., Rong, J., Zhang, M. et al. In vitro adventitious regeneration and plantlet transplantation of Atractylodes chinensis (DC.) Koidz., a valuable medicinal plant. Plant Cell Tiss Organ Cult 155, 209–220 (2023). https://doi.org/10.1007/s11240-023-02573-9
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DOI: https://doi.org/10.1007/s11240-023-02573-9