Abstract
The secondary metabolism of plants is affected by several factors, including the type of cultivation and light, which directly interfere with photosynthesis, causing changes in the content and composition of secondary plant metabolites. Thus, this study sought to answer whether the light lengths of red light emitting diodes (LED), as well as different cultivation methods, can cause changes in the chemical composition of the essential oil of Acmella oleracea. The experimental design was divided into four treatments. T1: in vitro using LED lights for indoor cultivation; T2: in vitro with red LED light; T3: in pot with LED lights for indoor cultivation and T4: in pot with red LED light. All treatments were kept in a biochemical oxygen demand oven for 45 days. Leaf material was collected, essential oil was extracted by hydrodistillation, with subsequent chemical characterization by gas chromatography coupled with mass spectrometry (GC/MS). The results showed that trans-cariophyllene (13.04% and 8.89%) and 2-tridecanone (16.45% and 15.09%) compounds were directly affected by red LED light, in both in vitro (T2) and in pot (T4) treatments. The major compound α-cadinol (18.75%) and 4-epi-cubedol (29.11%) were directly affected by the treatment T3. The results indicated that red light and cultivation methods significantly influenced the chemical composition of A. oleracea essential oil.
Key message
Red LED light affected the production of secondary metabolites in Acmella oleracea. Trans-cariophyllene and 2-tridecanone were affected by red LED light in both cultivation treatments.
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The authors are grateful to Paranaense University, Postgraduate Program in Biotechnology Applied to Agriculture of Paranaense University.
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This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Araucária.
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Dedino, DB: Conceptualization, Methodology, Writing- Original draft preparation; Lima, JD: Conceptualization, Methodology; Rivadavea, WR: Methodology; Bortolucci, W: Methodology, Data curation, Writing- Reviewing and Editing; Wietzikoski, EC: Writing- Reviewing and Editing; Gazim, ZC: Supervision, Validation, Writing-Reviewing and Editing Funding acquisition; Gonçalves, JE: Methodology, Resources, Funding acquisition; Monzon DLR: Writing – Reviewing and Editing; Silva, GJ: Conceptualization, Visualization, Writing- Reviewing and Editing, Resources, Funding acquisition.
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Dedino, D.B., de Lima, J.D., Bortolucci, W.d.C. et al. Red LED light and different cultivation methods changed the essential oil composition of Acmella oleracea. Plant Cell Tiss Organ Cult 151, 511–520 (2022). https://doi.org/10.1007/s11240-022-02367-5
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DOI: https://doi.org/10.1007/s11240-022-02367-5