Abstract
The influence of semi-continuous culture on root growth and production of saikosaponins was investigated using root cultures of Bupleurum chinense DC. When fresh medium, consisting of half-strength of Murashige and Skoog liquid medium and 5% sucrose, was added at day 30, numerous new young roots developed and grew. Also, an increase in root weight and the total amount of saikosaponins was observed at day 60. Both the concentration and time of adding sucrose to the culture medium had significant effects on root growth and production of total saikosaponins. The sucrose concentration was controlled by multiple additions of fresh sucrose at different time points during root culture. Compared to other reported methods, by which sucrose concentration was controlled in culture, sequential increases of 1% sucrose at days 5, 10, 15, and 20 promoted both root growth and the production of saikosaponins.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig2_HTML.jpg)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig3_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig4_HTML.jpg)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig5_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11240-011-0012-x/MediaObjects/11240_2011_12_Fig6_HTML.gif)
References
Chen SL, **ao PG (2006) The introduction of traditional chinese medicine resources. Medical Science and Technology Press, Bei**g
Claudia SG, Lĺvia da SC, Tatiana CC, Cátia HC, Norma A, Elisabeth M (2011) Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae). Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9945-3
Conner AJ, Falloon PJ (1993) Osmotic versus nutritional effects when root in vitro asparagus minicrowns on high sucrose media. Plant Sci 89:101–106
Cormier F, Crevier HA, Do CB (1990) Effects of sucrose concentration on the accumulation of anthocyanins in grape (Vitis vinifera) cell suspension. Can J Bot 68:1822–1825
Guan Q, Hao JP, Yang DF, Han D, Gao KQ, Zhou Q (2011) Biotransformation and biosynthesis of saikosaponins in adventitious root system of Bupleurum chinense. Nat Prod Res Dev 23(2):208–211
Hao JP, Xu LX, Yang DF (2008a) Condition for induction and differentiation of callus and propagation of adventitious buds in Bupleurum chinense. Chin Tradi herb Drugs 39(8):1234–1238
Hao JP, Xu XF, Yang DF, Hou J, Hu K, Qin XM (2008b) Rapid propagation and seed germinating condition of Bupleurum Chinense. Chin Herb Med 39(5):640–644
Hu YH, Yu YT, Piao CH, Liu JM, Yu HS (2011) Methyl jasmonate- and salicylic acid-induced D-chiro-inositol production in suspension cultures of buckwheat (Fagopyrum esculentum). Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9938-2
Kato A, Kawazoe S, Soh Y (1978) Viscosity of the broth of the tobacco cells in suspension culture. J Ferment Technol 56:224–228
Kusakari K, Yokoyama M, Inomata S (2000) Enhanced production of saikosaponins by root culture of Bupleurum falcatum L. using two-step control of sugar concentration. Plant Cell Rep 19:1115–1120
Li YY, Qin XM, Wang YQ, Yue JY, Hao JP (2008) Determination of content of saikoside in Bupleurum Chinense from different sources. China J Chin Materia Medica 33(3):237–240
Liu MX, Xu ZG, Yang Y, Feng YJ (2010) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tissue Organ Cult 106:1–10
Lu LX, Yu XB, **ong Q, **ong XH (2005) Study on high cell density culture of Lactobacillus sp. by semi-continuous way. Sci Technol Food Ind 12:47–49
Neusa M, Sandra G, Taˆnia P, Anabela R (2011) The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis. Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9963-1
Qu JG, Zhang W, Yu XJ (2011) A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera. Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9977-8
Rokem JS, Goberg I (1985) Secondary metabolites from plant tissue culture: methods for yield improvement. In: Mizrahi A, Van Wezel AL (eds) Advances in biotechnnological processes 4. Alan R. Liss, New York, pp 241–247
Saikat G, Nirmal M, Somnath B, Prakash KD (2011) Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9947-1
Sarkissian GK, Fowler MW (1986) The metabolism and utilization of carbohydrates by suspension cultures of plant cells. In: Morgan MJ (ed) Carbohydrate metabolism in cultured cells. Plenum Press, New York, pp 151–181
Suriyan C, Chaturong C, Watchara C, Aussanee P, Kanyaratt S (2011) Promoting root induction and growth of in vitro macadamia (Macadamia tetraphylla L. ‘Keaau’) plantlets using CO2-enriched photoautotrophic conditions. Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9940-8
Tanavat K, Puangpaka S, Noppamas S, Sompop P (2011) In vitro induction of polyploidy in Centella asiatica (L.) urban. Plant Cell Tissue Organ Cult (in press). doi:10.1007/s11240-011-9969-8
Xue JP, Liu J, Jiang XW (2006) Medicinal plant biotechnology. China University of Technology, Hefei
Yao Z, Gao WY, Li KF, Pake KY (2007) Callus growth and adventitious root induction in Bupleurum chinense. Chin Tradi Herb Drugs 38(2):275–278
Zhong JJ, Yoshida T (1995) High density cultivation of Perilla frutescens cell suspensions for anthocyanin production: effect of sucrose concentration and inoculum size. Enzyme Microb Technol 17:1073–1079
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hao, Jp., Guan, Q. Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture. Plant Cell Tiss Organ Cult 108, 159–165 (2012). https://doi.org/10.1007/s11240-011-0012-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-011-0012-x