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Liquid overlaying improves somatic embryogenesis in Catharanthus roseus

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Abstract

Somatic embryos were induced from in vitro germinating seed-hypocotyls of Catharanthus roseus. The process of embryogenesis has been categorized into a few distinct stages (induction, proliferation, maturation and germination) in which liquid overlaying at varying levels 0 ml (T0), 0.25 ml (T1), 0.5 ml (T2), 0.75 ml (T3) and 1.0 ml (T4) was applied on solid medium. It was found that liquid overlaying improved proliferation; maturation, germination of embryos in C. roseus. In proliferation stage, particularly in T2, torpedo embryo number increased significantly (i.e. 129.6%) as compared to control. Liquid overlaying (T2, T3 and T4) also improved embryo maturation and showed early germination even in maturation medium. It also accelerated normal embryo germination frequency particularly in treatment with T2 and shortened ‘embryo—plantlet’ recovery time. Biochemical analyses revealed more proline, protein and amino acid with increasing level of liquid overlaying as it improved embryo induction, development and faster germination.

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Abbreviations

ANOVA:

Analysis of variance

BA:

N6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

KIN:

Kinetin

NAA:

Naphthalene acetic acid

MS:

Murashige and Skoog (1962) medium

TCA:

Trichloroacetic acid

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Acknowledgments

The first author is thankful to Council of Scientific and Industrial Research (CSIR) for receiving Senior Research Fellowship (SRF). Thanks are also due to Dr. Saumitra Banerjee for his help with this manuscript.

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Authors and Affiliations

  1. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi, 110062, India

    Zahid H. Siddiqui, A. Mujib & Mehpara Maqsood

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  1. Zahid H. Siddiqui
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  2. A. Mujib
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  3. Mehpara Maqsood
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Correspondence to A. Mujib.

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Siddiqui, Z.H., Mujib, A. & Maqsood, M. Liquid overlaying improves somatic embryogenesis in Catharanthus roseus . Plant Cell Tiss Organ Cult 104, 247–256 (2011). https://doi.org/10.1007/s11240-010-9828-z

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  • DOI: https://doi.org/10.1007/s11240-010-9828-z

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