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Physicochemical and Antioxidant Properties of Industrial Hemp Seed Protein Isolate Treated by High-Intensity Ultrasound

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Abstract

Ultrasound is one of the non-thermal, green, and novel technologies used to functionalize plant proteins. We recently determined the optimum conditions of high-intensity ultrasound (HIUS) treatment for maximum solubility and investigated the functional properties of hemp seed protein isolate (HSPI) under the optimal conditions. In this study, we analyzed changes in primary, secondary, and tertiary structures, physical microstructures, thermal stability, and antioxidant capacity of ultrasound-applied hemp protein isolate (HSPI-HIUS). The free SH group content (+59%) and zeta potential (+25%) increased upon ultrasound treatment. The electrophoretic protein patterns of HSPI showed no significant change after HIUS treatment. The FTIR spectrum revealed the wavenumber shifts in Amid 1 and 2 regions of protein. The denaturation temperature and the ratio of β-structure increased after sonication. Antioxidant properties of hemp seed protein isolates were increased by 38% by ultrasound treatment. The obtained data in this study showed that HIUS treatment would be promising for improving the functional, physicochemical, and antioxidant properties of HSPI.

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Acknowledgments

This study was the second part of Mrs. Karabulut’s Ph. D. thesis. The authors would also like to acknowledge the valuable help of Yavuz Derin during FTIR analysis.

Funding

This work was supported by the Commission of Scientific Research Projects of Sakarya University (Sakarya, Turkey) [Grant Number 2020–7–25–24].

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Authors and Affiliations

  1. Faculty of Engineering, Department of Food Engineering, Sakarya University, 54187, Sakarya, Turkey

    Gulsah Karabulut & Oktay Yemiş

  2. Department of Food Science and Human Nutrition, Illinois University at Urbana Champaign, Urbana, IL, 61801, USA

    Gulsah Karabulut & Hao Feng

  3. Research, Development and Application Center (SARGEM), Sakarya University, 54050, Sakarya, Turkey

    Gulsah Karabulut & Oktay Yemiş

Authors
  1. Gulsah Karabulut
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  2. Hao Feng
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Contributions

Gulsah KARABULUT: Methodology, Writing-Original draft, Formal analysis. Hao FENG: Reviewing, Editing. Oktay YEMİŞ: Funding Acquisition, Supervision, Critical Reviewing-Editing.

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Correspondence to Oktay Yemiş.

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Karabulut, G., Feng, H. & Yemiş, O. Physicochemical and Antioxidant Properties of Industrial Hemp Seed Protein Isolate Treated by High-Intensity Ultrasound. Plant Foods Hum Nutr 77, 577–583 (2022). https://doi.org/10.1007/s11130-022-01017-7

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