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iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Tobacco Callus Response to Carbon Nanoparticles

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Abstract

Carbon nanomaterials (CNMs) have attracted a great deal of research interest for their potential application in plants. Some types of CNMs have exhibited the ability on regulating plant growth of plants, which showed a promising future in agriculture. However, detailed mechanism of their regulation on plant growth is still not well characterized especially at the molecular level. In this study, our objective is to study the regulation mechanism of carbon nanoparticles (CNPs) on tobacco callus growth at protein level. During the incubation period, proteomic profiling of tobacco callus was investigated by using the isobaric tags for relative and absolute quantitation labelling (iTRAQ) coupled with 2D-LC MS/MS. The function of differentially expressed proteins was achieved by using a gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis with InterproScan, and qRT-PCR was used to support the results of the proteomic data. The results showed that the growth of tobacco callus was enhanced by CNPs, with the optimal concentration at 50 mg/L. The expression levels of 198 cellular proteins were significantly up- or down-regulated after CNPs treatment. Some differentially expressed proteins were located in mitochondria and involved in calcium-mediated signaling pathway. The proteomic profile changes provided more deeper understanding of the highly complex regulatory mechanisms in tobacco callus exposed to CNPs.

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Abbreviations

CNMs:

Carbon nanomaterials

CNPs:

Carbon nanoparticles

GO:

Gene ontology

iTRAQ:

Isobaric tag for relative and absolute quantitation

KEGG:

Kyoto encyclopedia of genes and genomes

TBY-2 cell:

Nicotiana tabacum L. Bright Yellow-2 cell

TEM:

Transmission electron microscopy

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Funding

The work described in this paper was substantially supported by Zhengzhou Tobacco Research Institute Dean Fund (112017CA0090) and Science and Technology Project of China Tobacco Corporation (110201902004).

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Authors and Affiliations

  1. Technology Center, China Tobacco Zhejiang Industrial Corporation, Hangzhou, China

    Zhenjie Zhao

  2. Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, China

    Zhenjie Zhao, Liwei Hu, Qiansi Chen, Huaxin Dai, Qisheng Yin & Taibo Liang

  3. Golden-Leaf Production Manufacturing Center, China Tobacco Henan Industrial Corporation, Zhengzhou, China

    **angyu Meng

Authors
  1. Zhenjie Zhao
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  2. Liwei Hu
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  3. Qiansi Chen
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  4. Huaxin Dai
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  5. **angyu Meng
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  6. Qisheng Yin
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  7. Taibo Liang
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Contributions

QY and TL conceived and designed the experiments. ZZ, QC, and XM performed the experiments. ZZ, LH, and HD participated in the data analysis. ZZ and TL drafted the manuscript. TL provided guidance for preparing the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Taibo Liang.

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Key Message

• CNPs induced growth enhancement of tobacco callus. Proteins regulated by CNPs were identified by iTRAQ-based quantitative proteomics, and the expression levels of 198 cellular proteins were significantly up- or down-regulated. The differentially expressed proteins were located in mitochondria and involved in calcium-mediated signaling pathway.

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Zhao, Z., Hu, L., Chen, Q. et al. iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Tobacco Callus Response to Carbon Nanoparticles. Plant Mol Biol Rep 40, 556–565 (2022). https://doi.org/10.1007/s11105-022-01337-7

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  • DOI: https://doi.org/10.1007/s11105-022-01337-7

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