Abstract
Background and aims
Dissolved organic matter (DOM) has a key role in soil biogeochemistry and is vulnerable to global climate change such as drought stress. Intercrop** provides greater bioavailable resources and soil carbon inputs, compared to monoculture. However, little data exist on the DOM quantity and quality of intercrop** soil responses to drought.
Methods
We conducted a two-year manipulated rainfall-reduction field experiment including water (the control and drought), and crop** system (millet/peanut intercrop** and corresponding monoculture). After crop harvesting, DOM contents and compositions were determined and characterized using ultraviolet–visible absorbance and fluorescence spectroscopy.
Results
In 0–20 cm, DOC contents were reduced by 44.3% in intercrop** and 23.8% under drought. However, the specific ultraviolet absorbance at 254 nm (SUVA254) and 260 nm (SUVA260) of DOM were increased in intercrop**, indicating greater aromatic and hydrophobic DOM in intercrop** soil, and the enhanced effect was observed under drought. Four identified DOM components were UVC humic-like (C1), microbial-derived humic-like (C2), unidentified humic-like (C3), and tyrosine-like (C4). In detail, C1, C2, C3, and C4 were all higher in intercrop**, especially under drought treatment (P < 0.05). Humification index (HIX), fluorescence index (FI), and biological index (BIX) indicated mixed DOM sources (terrestrially-derived and microbial-derived), FI and BIX were higher under drought.
Conclusion
Although drought decreased DOC content, the DOM components in intercrop** could be more stable due to their higher aromaticity and hydrophobicity. These findings provide a new theoretical basis to evaluate soil fertility in various agroecosystems and understand how soil DOM responding to drought.
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Data Availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We would like to thank Dr. Ruipeng Yu, Hao Yang, and Ran An for their help in the statistical analysis.
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This study was financially supported by the National Natural Science Foundation of China (42077063, 31700366) and the Fundamental Research Funds for the Central Universities (2020TC118).
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Huasen Xu and **ang Wang contributed to the study conception and design. Material preparation, data collection and analysis were performed by Yumei Peng, Zi Wang, Jia Shi and Junfei Lv. The first draft of the manuscript was written by Yumei Peng and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Peng, Y., Xu, H., Wang, Z. et al. Responses of the content and spectral characteristics of dissolved organic matter in intercrop** soil to drought in northeast China. Plant Soil (2023). https://doi.org/10.1007/s11104-023-05931-w
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DOI: https://doi.org/10.1007/s11104-023-05931-w