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Visualization of grapevine root colonization by the Saharan soil isolate Saccharothrix algeriensis NRRL B-24137 using DOPE-FISH microscopy

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Abstract

Background and aim

There is currently a gap of knowledge regarding whether some beneficial bacteria isolated from desert soils can colonize epi- and endophytically plants of temperate regions. In this study, the early steps of the colonization process of one of these bacteria, Saccharothrix algeriensis NRRL B-24137, was studied on grapevine roots to determine if this beneficial strain can colonize a non-natural host plant. An improved method of fluorescence in situ hybridization (FISH), the double labeling of oligonucleotide probes (DOPE)-FISH technique was used to visualize the colonization behavior of such bacteria as well as to determine if the method could be used to track microbes on and inside plants.

Methods

A probe specific to Saccharothrix spp. was firstly designed. Visualization of the colonization behavior of S. algeriensis NRRL B-24137 on and inside roots of grapevine plants was then carried out with DOPE-FISH microscopy.

Results

The results showed that 10 days after inoculation, the strain could colonize the root hair zone, root elongation zone, as well as root emergence sites by establishing different forms of bacterial structures as revealed by the DOPE-FISH technique. Further observations showed that the strain could be also endophytic inside the endorhiza of grapevine plants.

Conclusions

Taking into account the natural niches of this beneficial strain, this study exemplifies that, in spite of its isolation from desert soil, the strain can establish populations as well as subpopulations on and inside grapevine plants and that the DOPE-FISH tool can allow to detect it.

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Acknowledgments

The authors are grateful to the Higher Education Commission of Pakistan for according PhD scholarships to Ms. Saima Muzammil. Additional thanks for the platform of microscopy of INRA Castanet-Tolosan (France) for allowing us the use of microtome and glass knives. We would like also to acknowledge Dr. Günter Brader and Ms. Helen Smith (AIT, Austria) for proof reading of the manuscript.

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  1. Stéphane Compant

    Present address: Bioresources Unit, Health & Environment Department, AIT Austrian Institute of Technology GmbH, 3430, Tulln, Austria

Authors and Affiliations

  1. LGC UMR 5503 (CNRS/INPT/UPS), Département Bioprocédés et Systèmes Microbiens, ENSAT-INP de Toulouse, Université de Toulouse, 1 Avenue de l’Agrobiopôle, B.P. 32607, 31326, Castanet-Tolosan Cedex 1, France

    Stéphane Compant, Saima Muzammil, Ahmed Lebrihi & Florence Mathieu

  2. Université Moulay Ismail, Marjane II, BP 298, Meknès, Morocco

    Ahmed Lebrihi

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  1. Stéphane Compant
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Correspondence to Stéphane Compant.

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Responsible Editor: Jesus Mercado-Blanco.

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Compant, S., Muzammil, S., Lebrihi, A. et al. Visualization of grapevine root colonization by the Saharan soil isolate Saccharothrix algeriensis NRRL B-24137 using DOPE-FISH microscopy. Plant Soil 370, 583–591 (2013). https://doi.org/10.1007/s11104-013-1648-6

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