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A type-2C protein phosphatase (GhDRP1) participates in cotton (Gossypium hirsutum) response to drought stress

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Abstract

Key message

GhDRP1 acts as a negatively regulator to participate in response to drought stress possibly by modulating ABA signaling pathway and flavonoid biosynthesis pathway which affects stomata movement and thus water loss, ROS scavenging enzymes, and proline accumulation in cotton.

Abstract

Type-2C protein phosphatases (PP2C) may play important roles in plant stress signal transduction. Here, we show the evidence that a cotton PP2C protein GhDRP1 participates in plant response to drought stress. GhDRP1 gene encodes an active type-2C protein phosphatase (PP2C) and its expression is significantly induced in cotton by drought stress. Compared with wild type, the GhDRP1 overexpression (OE) transgenic cotton and Arabidopsis displayed reduced drought tolerance, whereas GhDRP1-silenced (RNAi) cotton showed enhanced drought tolerance. Under drought stress, malondialdehyde content was lower, whereas superoxide dismutase and peroxidase activities, proline content, stomata closure and relative water content were higher in GhDRP1 RNAi plants compared with those in wild type. In contrast, GhDRP1 OE plants showed the opposite phenotype under the same conditions. Expression levels of some stress-related and flavonoid biosynthesis-related genes were altered in GhDRP1 transgenic plants under drought stress. Additionally, GhDRP1 protein could interact with other proteins such as PYLs, SNF1-related protein kinase and GLK1-like protein. Collectively, these data suggest that GhDRP1 participates in plant response to drought stress possibly by modulating ABA signaling pathway and flavonoid biosynthesis pathway which affects stomata movement and thus water loss, ROS scavenging enzymes, and proline accumulation in cotton.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Grant No. 31871667, 32001594), and the Project from the Ministry of Agriculture of China for transgenic research (Grant No. 2014ZX0800927B).

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  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China

    Yun Chen, **g-Bo Zhang, Ning Wei, Zhi-Hao Liu, Yang Li, Yong Zheng & Xue-Bao Li

  2. School of Life Sciences, Hubei Normal University, Huangshi, 435002, China

    Yun Chen & Zhi-Hao Liu

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  1. Yun Chen
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Contributions

X-BL and YC conceived and designed the research, YC, J-BZ, NW, Z-HL and YL performed the experiments, Y.C., Y.Z. and X.-B.L. analyzed the data, Y.C. and X.-B.L wrote the paper.

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Correspondence to Xue-Bao Li.

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Supplementary material 1 (PDF 1337.0 kb)

11103_2021_1198_MOESM2_ESM.xls

Dataset S1. Summary of the genes up-regulated in leaves of wild type (WT), GhDRP1OE and GhDRP1RNAi plants under normal and drought conditions by RNA-seq assay. Supplementary material 2 (XLS 1127.0 kb)

11103_2021_1198_MOESM3_ESM.xls

Dataset S2. Summary of the genes down-regulated in leaves of wild type (WT), GhDRP1OE and GhDRP1RNAi plants under normal and drought conditions by RNA-seq assay. Supplementary material 3 (XLS 1216.5 kb)

11103_2021_1198_MOESM4_ESM.xls

Dataset S3. List of 2096 genes that show similar expression patterns in wild type (WT), GhDRP1 OE and GhDRP1 RNAi plants under drought treatments, respectively. Supplementary material 4 (XLS 737.5 kb)

11103_2021_1198_MOESM5_ESM.xls

Dataset S4. List of 585 genes that both responded to drought stress and were affected by GhDRP1. Supplementary material 5 (XLS 432.0 kb)

11103_2021_1198_MOESM6_ESM.xls

Dataset S5. List of 25 genes that show opposite expression tendency in cotton leaves under normal conditions and drought treatments between GhDRP1 OE and RNAi transgenic cotton plants. Supplementary material 6 (XLS 37.5 kb)

Dataset S6. The raw data of quantitative RT-PCR experiments. Supplementary material 7 (XLS 288.5 kb)

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Chen, Y., Zhang, JB., Wei, N. et al. A type-2C protein phosphatase (GhDRP1) participates in cotton (Gossypium hirsutum) response to drought stress. Plant Mol Biol 107, 499–517 (2021). https://doi.org/10.1007/s11103-021-01198-w

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