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Establishing a papillary craniopharyngioma cell line by SV40LT-mediated immortalization

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Abstract

Background

Craniopharyngioma represents a troublesome tumor of the intracranial sellar region. There are currently no available well-characterized craniopharyngioma cell lines. This lack of reliable, immortal cell lines is a major reason for the slow progress in fundamental research related to craniopharyngioma.

Methods

We describe the development of an immortal papillary craniopharyngioma (PCP) cell line by transfecting primary PCP cells with the pLenti-simian virus 40 large T antigen(SV40LT).

Results

Three clones have been cultured for more than 14 months so far, while non-transfected cells ceased proliferation within three months of isolation. The established immortal PCP cell lines were identified to have BRAFV600E mutations, while no mutations in tumor suppressor genes were found in primary cells or immortal cells. Immortal cells had higher proliferation rates and formed tumors when implanted in the bran of nude mice. BRAF inhibition in immortal PCP cells altered cell morphology, inhibited cell proliferation and promoted apoptosis.

Conclusion

We successfully developed PCP cell lines by SV40LT-mediated immortalization. These cell lines represent a powerful tool for fundamental and therapeutical studies on craniopharyngioma.

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Data availability

Data sharing not applicable to this article as no datasets were generated or analysed during the current study.

Abbreviations

CP:

Craniopharyngioma

aCP:

Adamantinomatous craniopharyngioma

pCP:

Papillary craniopharyngioma

HE:

Hematoxylin and eosin

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Funding

This work is supported by grants from the Science and Technology Program of Guangdong (2016A020213006, 2017A020215048, and 2017A020215191); the Natural Science Foundation of Guangdong (2016A030310377); the Science and Technology Program of Guangzhou (201707010149); and the President Foundation of Nanfang Hospital, Southern Medical University (2015C018, 2016L002, and 2017Z009).

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Author notes

  1. Yi Liu, Chao-hu Wang and Jun Fan have contributed equally to this study.

Authors and Affiliations

  1. Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, 510515, China

    Yi Liu, Chao-hu Wang, Jun Fan, Jun-xiang Peng, Jun Pan, **’an Zhang & Song-tao Qi

Authors
  1. Yi Liu
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  2. Chao-hu Wang
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  3. Jun Fan
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  4. Jun-xiang Peng
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  5. Jun Pan
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  6. **’an Zhang
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  7. Song-tao Qi
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Contributions

Yi Liu and Songtao Qi performed majority of the experiments and contributed to the experimental design. Chaohu Wang and Jun Fan performed the experiments. Chaohu Wang and Qianchao Zhu performed the immunohistochemical staining. Yi Liu, **’an Zhang and Jun Pan performed the histopathological analysis of the human ACP samples. Yi Liu wrote the manuscript.

Corresponding author

Correspondence to Song-tao Qi.

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Ethics approval

This study was performed in accordance with all applicable international, national, and institutional guidelines of the Nanfang Hospital for the care and use of animals. All study procedures involving human participants and animals were performed in accordance with the ethical standards of the Nanfang Hospital research committee and the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Liu, Y., Wang, Ch., Fan, J. et al. Establishing a papillary craniopharyngioma cell line by SV40LT-mediated immortalization. Pituitary 24, 159–169 (2021). https://doi.org/10.1007/s11102-020-01093-5

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