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Comparison of photosynthesis and antioxidative protection in Sedum album and Sedum stoloniferum (Crassulaceae) under water stress

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Photosynthetica

Abstract

Photosynthetic gas exchange, dry mass production, water relations and inducibility of crassulacean acid metabolism (CAM) pathway as well as antioxidative protection during the C3-CAM shift were investigated in Sedum album and Sedum stoloniferum from Crassulaceae under water stress for 20 days. Leaf relative water content (RWC), leaf osmotic and water potential decreased with increasing water stress in both studied species. Significant reduction in dry matter production and leaf thickness was detected only in S. stoloniferum after 20-d water stress. Δtitratable acidity and phosphoenolpyruvate carboxylase (PEPC) activity in S. album responded to drought at early stages of stress treatment, continued to increase throughout the entire stress period and reached levels 15 times higher than those in well-watered plants. In S. stoloniferum, however, both parameters responded later and after a transient increase declined again. In S. stoloniferum, in spite of increase by drought stress, net night-time CO2 assimilation was negative resembling a C3-like pattern of gas exchange. Catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) activities increased in plants subjected to mild water stress while declined as the stress became severe. Although malondialdehyde (MDA) content was higher in drought-stressed S. stoloniferum, the increase in the concentration of hydrogen peroxide (H2O2) that may act as a signal for C3-CAM transition was higher in S. album compared with S. stoloniferum. In drought-stressed plants, SOD activity showed a clear diurnal fluctuation that was more steadily expressed in S. album. In addition, such pattern was observed for CAT only in S. album. We concluded that temporal and diurnal fluctuation patterns in the activity of antioxidant enzymes depended on duration of drought stress and was related to the mode of photosynthesis and degree of CAM induction. According to our results, S. stoloniferum developed a low degree of CAM activity, e.g. CAM-cycling metabolism, under drought conditions.

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Abbreviations

APX:

ascorbate peroxidase

CAM:

crassulacean acid metabolism

CAT:

catalase

DM:

dry mass

E :

transpiration rate

g s :

stomatal conductance

H2O2 :

hydrogen peroxide

MDA:

malondialdehyde

PEPC:

phosphoenolpyruvate carboxylase

P N :

net photosynthetic rate

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

Ψs :

osmotic potential

Ψw :

water potential

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Authors and Affiliations

  1. Department of Biology, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran

    G. Habibi

  2. Plant Science Department, University of Tabriz, 51666-14779, Tabriz, Iran

    R. Hajiboland

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  1. G. Habibi
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  2. R. Hajiboland
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Correspondence to G. Habibi.

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Habibi, G., Hajiboland, R. Comparison of photosynthesis and antioxidative protection in Sedum album and Sedum stoloniferum (Crassulaceae) under water stress. Photosynthetica 50, 508–518 (2012). https://doi.org/10.1007/s11099-012-0066-y

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  • DOI: https://doi.org/10.1007/s11099-012-0066-y

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