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Tetrachloromethane Induced Acute Liver Injury in Mice: Biochemical and Histopathological Study of the Hepatoprotective Effect of Hep-X Standardized Botanical Dietary Supplement

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Pharmaceutical Chemistry Journal Aims and scope

This work was aimed at investigating the hepatoprotective effect of Hep-X, a polyherbal formulation containing Silybum marianum L. (Milk thistle), Rosmarinus officinalis L. (Rosemary), Curcuma longa L. (Turmeric) and Fumaria officinalis L. (Fumitory) as standardized botanical dietary supplement, on mice with CCl4-induced acute liver injury. The total phenolic and flavonoid contents of Hep-X were determined as 0.125 and 0.528 mg/mL, respectively. The quercetin content was determined as 50 µg/mL using HPLC analysis. The total antioxidant capacity showed correlation between the Hep-X concentration and percentage inhibition of free radicals. Hep-X was administered orally at 25, 50 and 100 mg/kg b.w./day against CCl4-induced hepatotoxicity in mice. The hepatic damage was measured using blood biochemical parameters. Animals upon Hep-X treatment exhibited better drug effIcacy in certain blood parameters than silymarin-treated mice. Also, Hep-X administration significantly ameliorated the liver damage by suppressing iNOS expression and apoptosis as well as by recovery of the histological structure. The obtained results suggest that Hep-X is able to significantly alleviate the hepatotoxicity induced by CCl4 in mice, which can be due to antioxidant properties of the polyherbal formulation.

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References

  1. N. Kaplowitz, Sem. Liver Dis., 22, 137 (2002).

    Article  CAS  Google Scholar 

  2. N. Singh, V. Kamath, K. Narasimhamurthy, and P. S. Ra**i, Environ. Toxicol. Pharmacol., 26(2), 241 – 246 (2008).

    Article  CAS  PubMed  Google Scholar 

  3. A. Aslan, Ö. Gok, O. Erman, and T. Kuloglu, Biomed. Pharmacother., 105, 662 – 669 (2018).

    Article  CAS  PubMed  Google Scholar 

  4. J. A. Brent and B. H. Rumack, Clin. Toxicol., 3, 139 – 71 (1993).

    Google Scholar 

  5. L. Bergendi, L. Benes, Z. Duracková, and M. Ferencik, Life Sci., 65, 1865 – 74 (1999).

    Article  CAS  PubMed  Google Scholar 

  6. T. Jawaid, R. Patel, and P. Gautam, Int. J. Med. Pharm. Sci., No. 2, 1 – 9 (2011).

  7. T. Martinelli, F. Fulvio, M. Pietrella, et al., Fitoterapia, 148, 104797 (2021).

    Article  CAS  PubMed  Google Scholar 

  8. A. Kurkin, Pharm. Chem. J., 37, 189 – 202 (2003).

    Article  CAS  Google Scholar 

  9. N. E. Thomas-Eapen, Explore, 5(2), 114 – 115 (2009).

    Article  PubMed  Google Scholar 

  10. M. R. Al-Sereitia, K. M. Abu-Amer, and P. K. M. Sen, Indian J. Exp. Biol., 37, 124 – 131 (1999).

    Google Scholar 

  11. A. A. N. Aziz, R. Hasham R. M. Sarmidi, et al., Saudi Pharm. J., 29, 143 – 165 (2021).

  12. A. Meda, E. C. Lamien, M. Romito, et al., Chemistry, 91, 571 – 577 (2005).

    CAS  Google Scholar 

  13. C. C. Chang, H. M. Yang, H. M. Wen, and J. C. Chern, J. Food Drug Anal., 10, 178 – 82 (2002).

    CAS  Google Scholar 

  14. Z. Spacil, L. Novakova, and P. Solich, Talanta, 76, 189 – 199 (2008).

    Article  CAS  PubMed  Google Scholar 

  15. M. S. Blois, Nature, 181, 1199 – 200 (1958).

    Article  CAS  Google Scholar 

  16. T. Miyazawa, T. Suzuki, K. Fujimoto, T. Kaneda, J. Biochem., 107, 689 – 693 (1990).

    Article  CAS  PubMed  Google Scholar 

  17. Y. Shibayama, J. Pathol. 159(4), 335 – 339 (1989).

    Article  CAS  PubMed  Google Scholar 

  18. N. U. Karabay Yavaþoðlu, Ç. Köksal, M. Daðdeviren, et al., Environ. Toxicol., 29(3), 345 – 353 (2014).

  19. J. Quan, T. Li, W. Zhao, H. Xu, et al., J. Clin. Biochem. Nutr., 52(3), 244 – 252 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. D .L. Diesen and P. C. Kuo, J. Surg. Res. 162(1), 95–109 (2010).

    Article  CAS  PubMed  Google Scholar 

  21. S .L. Jothy, A. Aziz, Y. Chen, and S. Sasidharan, Evid. Based Complement. Alternat. Med., 1 – 10 (2012).

  22. A. Dechakhamphu and N. Wongchum, Asian Pac. J. Trop. Biomed., 5(12), 1042 – 1045 (2015).

    Article  Google Scholar 

  23. N. S. V. Bhujun, N. Munogee, and V. Coolen, J. Herb. Med., 4, 1 – 9 (2014).

    Article  Google Scholar 

  24. L. **-Wei, S. Ding, and X. Ding, Process Biochem., 40, 3607 – 13 (2005).

    Article  Google Scholar 

  25. M. Kifayatullah, S. M. Mustafa, P. Senguptha, et al., J. Acute Dis., 14, 1 – 7 (2015).

    Google Scholar 

  26. D. R. Dufour, J. A. Lott, F. S. Nolte, et al., Clin. Chem., 46, 2027 – 49 (2000)

    Article  CAS  PubMed  Google Scholar 

  27. S. K. Ramaiah, Food Chem. Toxicol. 45(9), 1551 – 1557 (2007).

    Article  CAS  PubMed  Google Scholar 

  28. A. Singh, T. K. Bhat, and P. O. Sharma, J. Clin. Toxicol., No. 4, 1 – 19 (2011).

  29. B. R. Thapa and A. Walia, Indian J. Pediatr., 74, 663 – 671 (2007).

    CAS  Google Scholar 

  30. S. F. Friedman, P. Martin, and J. S. Munoz, Laboratory evaluation of the patient with liver disease, in: Hepatology, a Textbook of Liver Disease, Philedelphia (2003), Vol. 1, pp. 661 – 709.

  31. L. Trapani, M. Segatto, and V. Pallottini, World J. Hepatol., 4, 184 – 190 (2012).

    Google Scholar 

  32. Y. J. Surh, K. S. Chun, H. H. Cha, et al., Mutat. Res. Fund. Mol. Mech. Mutagen., 480, 243 – 268 (2001).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Ege University, Faculty of Science, Department of Biology, 35100, Bornova, , Izmir, Turkey

    Cinel Koksal Karayildirim & Nefise Ulku Karabay Yavasoglu

  2. Sinop University, Faculty of Health Science, Department of Occupational Health, Sinop, Turkey

    Adem Guner

  3. Mugla Sitki Kocman University, Faculty of Medicine, Department of Histology and Embryology, Mugla, Turkey

    Gurkan Yigitturk

  4. Ege University, Center for Drug Research and Development and Pharmacokinetic Application, 35100, Bornova, , Izmir, Turkey

    Iskender Ince & Nefise Ulku Karabay Yavasoglu

  5. Naturin Nutraceuticals Products, Drug and Drug Raw Material Ltd, Izmir, Turkey

    Mustafa Yasar

  6. Ege University, Faculty of Medicine, Department of Histology and Embryology, 35100, Bornova, , Izmir, Turkey

    Altug Yavasoglu

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  1. Cinel Koksal Karayildirim
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  2. Adem Guner
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Correspondence to Nefise Ulku Karabay Yavasoglu.

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Karayildirim, C.K., Guner, A., Yigitturk, G. et al. Tetrachloromethane Induced Acute Liver Injury in Mice: Biochemical and Histopathological Study of the Hepatoprotective Effect of Hep-X Standardized Botanical Dietary Supplement. Pharm Chem J 56, 1639–1649 (2023). https://doi.org/10.1007/s11094-023-02839-8

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  • DOI: https://doi.org/10.1007/s11094-023-02839-8

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