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Circular RNA Circ_ANKMY2 Regulates Temporal Lobe Epilepsy Progression via the miR-106b-5p/FOXP1 Axis

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Abstract

Temporal lobe epilepsy (TLE) is common intractable epilepsy that affects the patient’s lives. The circular RNA circ_ANKMY2 (circ_ANKMY2) has been reported to be abnormally expressed in TLE. Nevertheless, the role and mechanism of circ_ANKMY2 in TLE are unclear. A human neuroblastoma cell line (SK-N-AS) was used for a series of studies. Expression levels of circ_ANKMY2, miR-106b-5p, and Forkhead Box Protein 1 (FOXP1) mRNA in TLE tissues were assessed through quantitative real-time polymerase chain reaction (qRT-PCR). Cell colony formation, proliferation, and apoptosis were determined by cell colony formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), or flow cytometry assays. The levels of FOXP1 protein, Ki67, B cell lymphoma (Bcl-2), Bcl-2 Associated X (Bax), and Cleaved caspase-3 were evaluated by western blot analysis. The relationship between circ_ANKMY2 or FOXP1 and miR-106b-5p was verified with dual-luciferase reporter assay. We observed that circ_ANKMY2 and FOXP1 expression were reduced while miR-106b-5p expression was increased in TLE tissues. Overexpression of circ_ANKMY2 decreased spontaneous recurrent seizures (SRSs) in rat TLE model and blocked cell colony formation, proliferation, and induced cell apoptosis in SK-N-AS cells. Importantly, circ_ANKMY2 was verified as a sponge for miR-106b-5p. In addition, miR-106b-5p mimics abolished circ_ANKMY2 elevation-mediated effects on colony formation, proliferation, and apoptosis of SK-N-AS cells. Also, FOXP1 served as a target for miR-106b-5p. And FOXP1 silencing overturned the effects of miR-106b-5p inhibitors on the colony formation, proliferation, and apoptosis of SK-N-AS cells. In sum, circ_ANKMY2 modulated TLE advancement via regulation of FOXP1 expression through sponging miR-106b-5p, and circ_ANKMY2 might be an underlying target for the improvement of TLE.

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  1. Department of Neurology, The First People’s Hospital of Taizhou, No.218 Hengjie Road, Huangyan District, Taizhou, 318020, Zhejiang, China

    Qing Lin, **ying Chen, **an Zheng, Yi Zhang, **aoxiao Tao & Jiamei Ye

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  1. Qing Lin
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11064_2020_3151_MOESM1_ESM.tif

Supplementary material 1—Impact of circ_ANKMY2 inhibition on cell colony formation and proliferation of SK-N-AS cells. (a) After si-NC or si-circ_ANKMY2 transfection, the expression of circ_ANKMY2 in SK-N-AS cells was examined with qRT-PCR. (b and c) The colony formation and proliferation of SK-N-AS cells were evaluated by colony formation or MTT assays. GAPDH was used as an internal control for circ_ANKMY2. Data were displayed as mean ± standard deviation, which was obtained from 3 replicate experiments. *P < 0.05. (TIF 1116 kb)

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Lin, Q., Chen, J., Zheng, X. et al. Circular RNA Circ_ANKMY2 Regulates Temporal Lobe Epilepsy Progression via the miR-106b-5p/FOXP1 Axis. Neurochem Res 45, 3034–3044 (2020). https://doi.org/10.1007/s11064-020-03151-7

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