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SSeCKS is a Suppressor in Schwann Cell Differentiation and Myelination

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Abstract

Src-suppressed protein kinase C substrate (SSeCKS) plays an important role in the differentiation process. In regeneration of sciatic nerve injury, expression of SSeCKS decreases, mainly in Schwann cells. However, the function of SSeCKS in Schwann cells differentiation remains unclear. We observed that SSeCKS was decreased in differentiated Schwann cells. In long-term SSeCKS-reduced Schwann cells, cell morphology changed and myelin gene expression induced by cAMP was accelerated. Myelination was also enhanced in SSeCKS-suppressed Schwann cells co-culture with dorsal root ganglion (DRG). In addition, we found suppression of SSeCKS expression promoted Akt serine 473 phosphorylation in cAMP-treated Schwann cells. In summary, our data indicated that SSeCKS was a negative regulator of myelinating glia differentiation.

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Acknowledgments

The authors wish to thank Dr. Yuxiang Hu for helpful criticism and linguistic revision of the manuscript. This work was supported by the National Natural Science Foundation of China (No. 30300099, No. 30770488, and No. 30870320); Natural Science Foundation of Jiangsu province (No. BK2006547, No. BK2009156, No. BK2009157, and No. BK2009161); Health Project of Jiangsu Province (H200632); Special Research Grant (XK200723) for the Key Laboratory from the Department of Health, Jiangsu Province; The Society and Technology Grew Project of Nantong City. (S2008020).

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Authors and Affiliations

  1. Laboratory Center, Affiliated Hospital of Nantong University, and The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, 226001, Nantong, People’s Republic of China

    Yuhong Ji, Junling Yang, Huiming Wang & Aiguo Shen

  2. Department of Immunology, Medical College, Nantong University, 19 Qixiu Road, 226001, Nantong, People’s Republic of China

    Yuhong Ji, Tao Tao, Chun Cheng, Huiguang Yang, Youhua Wang, Haiou Liu & **nxing He

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  1. Yuhong Ji
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  2. Tao Tao
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  3. Chun Cheng
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  9. Huiming Wang
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  10. Aiguo Shen
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Correspondence to Huiming Wang or Aiguo Shen.

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Yuhong Ji and Tao Tao contributed equally to this work.

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Ji, Y., Tao, T., Cheng, C. et al. SSeCKS is a Suppressor in Schwann Cell Differentiation and Myelination. Neurochem Res 35, 219–226 (2010). https://doi.org/10.1007/s11064-009-0045-2

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  • DOI: https://doi.org/10.1007/s11064-009-0045-2

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