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Plasma-derived extracellular vesicles improve mice embryo development

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Abstract

Background

To investigate the effect of plasma-derived extracellular vesicles (EVs) or conventional medium in fertilization and early embryo development rate in mice.

Methods and results

MII oocytes (matured in vivo or in vitro conditions) were obtained from female mice. The extracellular vesicles were isolated by ultracentrifugation of plasma and were analyzed and measured for size and morphology by dynamic light scattering (DLS) and transmission electron microscopy (TEM). By western blotting analysis, the EVs proteins markers such as CD82 protein and heat shock protein 90 (HSP90) were investigated. Incorporating DiI-labeled EVs within the oocyte cytoplasm was visible at 23 h in oocyte cytoplasm. Also, the effective proteins in the early reproductive process were determined in isolated EVs by western blotting. These EVs had a positive effect on the fertilization rate (P < 0.05). The early embryo development (8 cell, morula and blastocyst stages) was higher in groups supplemented with EVs (P < 0.01).

Conclusion

Our findings showed that supplementing in vitro maturation media with EVs derived- plasma was beneficial for mice’s embryo development.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This study was supported by grants from the Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Grant Number: 63134).

Author information

Author notes

  1. Maryam Javadi

    Present address: Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran

Authors and Affiliations

  1. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Maryam Javadi, Mohammad Sadegh Gholami Farashah, Leila Roshangar & Jafar Rad Soleimani

  2. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Leila Roshangar & Jafar Rad Soleimani

  3. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Mohammad Sadegh Gholami Farashah

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  1. Maryam Javadi
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  4. Jafar Rad Soleimani
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Contributions

Maryam Javadi and Leila Roshangar designed this study. Maryam Javadi and Mohammad Sadegh Gholami Farashah wrote the main manuscript text. Maryam Javadi prepared figures and analyzed the collected data. Jafar Soleimani Rad and Leila Roshangar edited the main text. All authors reviewed the manuscript and replied to revise.

Corresponding author

Correspondence to Maryam Javadi.

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Ethical approval

All procedures performed in present study were approved by Tabriz University of Medical Sciences’ Committee on Animal Experimentation, ethical approval number: IR.TBZMED.VCR.REC.1398.289.

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The authors declare no conflict of interest.

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Javadi, M., Gholami Farashah, M., Roshangar, L. et al. Plasma-derived extracellular vesicles improve mice embryo development. Mol Biol Rep 51, 621 (2024). https://doi.org/10.1007/s11033-024-09500-x

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