Abstract
Background
Lung cancer, one of the most common oncological diseases worldwide, continues to be the leading cause of cancer-related deaths. The development of new approaches for lung cancer, which still has a low survival rate despite medical advances, is of great importance.
Methods and results
In this study, bee venom (BV), conditioned medium of MSCs isolated from dental follicles (MSC-CM) and cisplatin were applied at different doses and their effects on A549 cell line were evaluated. Dental follicles were used as a source of MSCs source and differentiation kits, and characterization studies (flow cytometry) were performed. Cell viability was measured by the MTT method and apoptosis was measured by an Annexin V-FITC/PI kit on flow cytometer. IC50 dose values were determined according to the 24th hour and were determined as 15.8 µg/mL for BV, 10.78% for MSC-CM and 5.77 µg/mL for cisplatin. IC50 values found for BV and MSC-CM were also given in combination and the effects were observed. It was found that the applied substances caused BV to decrease in cell viability and induced apoptosis in cells. In addition to the induction of apoptosis in BV, MSC-CM, and combined use, all three applications led to an increase in Bax protein expression and a decrease in Bcl-2 protein expression. The molecular mechanism of anticancer activity through inhibition of Bax and Bcl-2 proteins and the NF-κB signaling pathway may be suggested.
Conclusion
Isolated MSCs in our study showed anticancer activity and BV and MSC-CM showed synergistic antiproliferative and apoptotic effects.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We would like to thank Selcuk University Scientific Research Projects Coordination Office and The Scientific and Technological Research Council of Türkiye (TUBITAK). We also thank Muslu Kazim Korez for statistical support.
Funding
This research was supported by Selçuk University Scientific Research Projects Coordination Office (Project No: 21112006) and The Scientific and Technological Research Council of Türkiye (TUBITAK) 1002 Short Term R&D Funding Program (Project No: 222S178).
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FS performed all the experiments, data analysis and drafting the manuscript. HV was supervised the project, editing the manuscript and participated in the finalization of the manuscript. BO participated in statistical analysis, interpretation of the results and editing the manuscript. All authors read and approved the final version of the manuscript.
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The study was approved by Selcuk University Faculty of Medicine Ethics Committee (Number: 2021/05, Date: 10.03.2021).
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Sengul, F., Vatansev, H. & Ozturk, B. Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549). Mol Biol Rep 51, 2 (2024). https://doi.org/10.1007/s11033-023-09002-2
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DOI: https://doi.org/10.1007/s11033-023-09002-2