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Genome-wide identification and gene expression analysis of GHMP kinase gene family in banana cv. Rasthali

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Abstract

Background

The GHMP kinase gene family encompasses ATP-dependent kinases, significantly involved in the biosynthesis of isoprenes, amino acids, and metabolism of carbohydrates. Banana is a staple tropical crop that is globally consumed but known for high sensitivity to salt, cold, and drought stresses. The GHMP kinases are known to play a significant role during abiotic stresses in plants. The present study emphasizes the role of GHMP kinases in various abiotic stress conditions in banana.

Methods and results

We identified 12 GHMP kinase (MaGHMP kinase) genes in the banana genome database and witnessed the presence of the conserved Pro-X-X-X-Gly-Leu-X-Ser-Ser-Ala domain in their protein sequences. All genes were found to be involved in ATP-binding and carried kinase activity confronting their biological roles in the isoprene (27%) and amino acid (20%) biosyntheses. The expression analysis of genes during cold, drought, and salt stress conditions in tissue culture grown banana cultivar Rasthali plants showed a significant involvement of MaGHMP kinase genes in these stress conditions. The highest expression of MaGHMP kinase3 (8.5 fold) was noted during cold stress, while MaGHMP kinase1 (25 fold and 40.01 fold) showed maximum expression during drought and salt stress conditions in leaf tissue of Rasthali.

Conclusion

Our findings suggested that MaGHMP kinase1 (MaHSK) and MaGHMP kinase3 (MaGlcAK) could be considered promising candidates for thwarting the abiotic stresses in banana.

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Data Availability

All data have been presented in the manuscript.

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Acknowledgements

The authors are thankful to the National Agri-Food Biotechnology Institute (NABI), Mohali, for providing the support and facilities to conduct the research. The authors are also grateful to the DBT e-Library Consortium (DeLCON) for providing journal access and to BIRAC for banana biofortification project support to NABI. S.C. is thankful to the Panjab University, Chandigarh; S.K. is thankful to the Central University of Punjab, Bathinda; and A.J. is thankful to Guru Jambheshwar University of Science and Technology, Hisar, Haryana, for their Ph.D. registrations.

Funding

This work was supported by NABI Core grant and banana biofortification project supported by BIRAC. The funders had no role in the design of the study; in the collection, analyses, and interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Author notes

  1. Siddhant Chaturvedi

    Present address: Department of Botany, Goswami Tulsidas Government Post Graduate College (Bundelkhand University, Jhansi), Karwi, Chitrakoot, Uttar Pradesh, 210205, India

Authors and Affiliations

  1. Plant Tissue Culture and Genetic Engineering Lab, Department of Biotechnology, S.A.S. Nagar, Ministry of Science and Technology (Government of India), National Agri-Food Biotechnology Institute (NABI), Sector 81, Knowledge City, Mohali, Punjab, 140306, India

    Siddhant Chaturvedi, Shahirina Khan, Neha Thakur & Siddharth Tiwari

  2. Department of Biotechnology, Panjab University, Chandigarh, 160014, India

    Siddhant Chaturvedi

  3. Department of Botany, Central University of Punjab, Bathinda, Punjab, 151001, India

    Shahirina Khan

  4. Department of Bio and Nanotechnology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India

    Alka Jangra

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  1. Siddhant Chaturvedi
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Contributions

S.T. conceived the idea and experiments. S.C. and S.K. performed computational and qRT-PCR analysis. S.T., S.C., and A.J. analyzed the data. S.T. and S.C. wrote the manuscript. N.T. and A.J. helped in the editing of the manuscript. S.T. contributed towards the analysis and writing of the manuscript. All authors read and agreed to the final version of the manuscript.

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Correspondence to Siddharth Tiwari.

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Chaturvedi, S., Khan, S., Thakur, N. et al. Genome-wide identification and gene expression analysis of GHMP kinase gene family in banana cv. Rasthali. Mol Biol Rep 50, 9061–9072 (2023). https://doi.org/10.1007/s11033-023-08743-4

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