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Acute cold stress induces intestinal injury via CIRP–TLR4–IRE1 signaling pathway in pre-starter broilers

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Abstract

Background

Cold stress is a common environmental stress in broiler chicks. Cold-inducible RNA-binding protein (CIRP) is a conserved cold shock protein that can regulate inflammatory response through Toll-like receptor 4 (TLR4). The mechanism that how CIRP involves in the regulation of cold stress in broilers remains unclear.

Methods and results

In this study, 360 7-day-old healthy male SZ901 chicks were selected and randomly allocated to four groups, and then subjected to acute cold exposure at the ambient temperature of 12 ± 1 °C for 0 h, 4 h, 8 h, and 12 h, respectively. After cold exposure, abdominall skin temperature, gene expression of CIRP–TLR4–IRE1 signaling pathway in ileum mucosa, and small intestinal structure were measured. The results showed that cold exposure decreased abdominall skin temperature, upregulated the gene expression of endoplasmic reticulum stress (ERS) markers IRE1, inflammatory factors IL-1β, IL-6, IL-10, TNF-α, and tight junction proteins ZO-1 and Occludin in ileum of chicks compared with the control group with no (0 h) cold exposure. Compared with the control group, a long time cold exposure upregulated the gene expression of CIRP, TLR4, GRP78, NF-κB in ileum mucosa, and decreased the villus height and V/C of small intestine.

Conclusions

The above results suggest that acute cold stress induces endoplasmic reticulum stress via upregulating the gene expression of CIRP–TLR4–IRE1 signaling pathway, and results in the structural damage of chick intestine.

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Fig. 1

Data availability

The datasets used and/or analyzed during the present study may be obtained from the corresponding author on reasonable request.

Abbreviations

CIRP:

Cold-inducible RNA-binding protein

TLR4:

Toll-like receptor 4

SZ901:

Shengze 901, a new breed of white feather broiler

IRE1:

 Inositol-requiring kinase 1

GRP78:

 Glucose-regulated protein 78

XBP1:

X-box binding protein-1

NF-κB:

 Nuclear factor-kappa B

ERS:

Endoplasmic reticulum stress

IL-1β:

Interleukin-1 beta

IL-6:

Interleukin-6

IL-10:

Interleukin-10

TNF-α:

Tumor necrosis factor-α

ZO-1:

 Zonula occludens-1

V/C:

Villus height to crypt depth ratio

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Acknowledgements

Not applicable.

Funding

This work was supported by National Natural Science Foundation of China (32160799), Jiangxi Provincial Natural Science Foundation (20224ACB205010).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang, 330045, Jiangxi, China

    Juanjuan Wu, Guiyao Li, Haoneng Guo & Guanhong Li

  2. Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, 332005, Jiangxi, China

    Bo Huang & Sifa Dai

  3. Jiujiang Bozheng Institute of Biotechnology Industry, Jiujiang, 332005, Jiangxi, China

    Sifa Dai

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  1. Juanjuan Wu
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All authors have confrmed their participation in this study.

Corresponding author

Correspondence to Sifa Dai.

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This study was performed in line with the guidelines for the care and use of experimental animals of the Ministry of Science and Technology of the People’s Republic of China. Approval was granted by the Animal Care and Use Committee of Jiangxi Agriculture University.

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Wu, J., Li, G., Guo, H. et al. Acute cold stress induces intestinal injury via CIRP–TLR4–IRE1 signaling pathway in pre-starter broilers. Mol Biol Rep 50, 6299–6304 (2023). https://doi.org/10.1007/s11033-023-08487-1

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