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Characterization and expression analysis of basic leucine zipper (bZIP) transcription factors responsive to chilling injury in peach fruit

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Abstract

Background

Peach (Prunus persica L.) is prone to chilling injury as exhibited by inhibition of the ethylene production, failure in softening, and the manifestation of internal browning. The basic leucine zipper (bZIP) transcription factors play an essential role in regulatory networks that control many processes associated with physiological, abiotic and biotic stress responses in fruits. Formerly, the underlying molecular and regulatory mechanism of (bZIP) transcription factors responsive to chilling injury in peach fruit is still elusive.

Methods and results

In the current experiment, the solute peach ‘Zhongyou Peach No. 13’ was used as the test material and cold storage at low temperature (4 °C). It was found that long-term low-temperature storage induced the production of ethylene, the hardness of the pulp decreased, and the low temperature also induced ABA accumulation. The changes of ABA and ethylene in peach fruits during low-temperature storage were clarified. Since the bZIP transcription factor is involved in the regulation of downstream pathways of ABA signals, 47 peach bZIP transcription factor family genes were identified through bioinformatics analysis. Further based on RT-qPCR analysis, 18 PpbZIP genes were discovered to be expressed in refrigerated peach fruits. Among them, the expression of PpbZIP23 and PpbZIP25 was significantly reduced during the refrigeration process, the promoter analysis of these genes found that this region contains the MYC/MYB/ABRES binding element, but not the DRES/CBFS element, indicating that the expression may be regulated by the ABA-dependent cold induction pathway, thereby responding to chilling injury in peach fruit.

Conclusions

Over investigation will provide new insights for further postharvest protocols related to molecular changes during cold storage and will prove a better cope for chilling injury.

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Data availability

All supporting data is available within the text and supplementary files. Further queries can be directed to corresponding author.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China [No. 31872085], the National Key Research and Development Program [2018YFD1000200], and the Agricultural Science and Technology Innovation Program (ASTIP) [CAAS-ASTIP-2019-ZFRI]. Authors are highly grateful for providing financial assistance for this research.

Funding

This work was supported by the National Natural Science Foundation of China [No. 31872085], the National Key Research and Development Program [2018YFD1000200], and the Agricultural Science and Technology Innovation Program (ASTIP) [CAAS-ASTIP-2019-ZFRI].

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Authors and Affiliations

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, People’s Republic of China

    Muhammad Muzammal Aslam, Li Deng, Junren Meng, Yan Wang, Lei Pan, Liang Niu, Zhenhua Lu, Guochao Cui, Wenfang Zeng & Zhiqiang Wang

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  1. Muhammad Muzammal Aslam
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Contributions

WZ conceived this project and designed all the experiment. MMA, LD, YW performed the experiments. JM analyzed data. ZW supervised the experiments MMA and WZ wrote the article, LP, LN, ZL and GC help to revise the manuscript.

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Correspondence to Wenfang Zeng or Zhiqiang Wang.

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Aslam, M.M., Deng, L., Meng, J. et al. Characterization and expression analysis of basic leucine zipper (bZIP) transcription factors responsive to chilling injury in peach fruit. Mol Biol Rep 50, 361–376 (2023). https://doi.org/10.1007/s11033-022-08035-3

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