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Development and characterization of microsatellite markers in the African timber tree species Cylicodiscus gabunensis (Fabaceae)

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Abstract

Background

Cylicodiscus gabunensis (Fabaceae) or ‘Okan’ is a Central African multipurpose timber species that is used for heavy construction and traditional medicine. Despite being currently heavily exploited, the species shows a low population density and a natural regeneration deficit in dense forest.

Methods and Results

We aimed to characterize polymorphic microsatellite markers that can be used to study patterns of genetic structure and gene flow (mating system, pollen and seed dispersal and inbreeding depression) and ultimately, help to develop sustainable forest management practices. We developed 24 polymorphic markers that can be amplified in three PCR multiplexes that were tested in 647 individuals of C. gabunensis from three populations. The number of alleles ranged from 4 to 27 and the average observed and expected heterozygosity across loci and populations were Ho = 0.585 (0.081–0.936) and He = 0.510 (0.076–0.914), respectively.

Conclusions

This set of markers is a useful tool for exploring intra-specific diversity, genetic structure and gene flow of C. gabunensis.

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Acknowledgements

We thank E. Kaymak for her help in the laboratory. We also thank J.-F. Gillet, D. Vanderhoeven, A. Boubady, and S.B. Ango for their help with data collection.

Funding

We thank the “Fonds pour la Formation à la Recherche dans l’Industrie et l’Agriculture” (FRIA, Belgium) for providing a PhD Grant to OB (FRIA/FC 27793). Molecular analyses were funded by the F.R.S-FNRS WISD Grant X.3040.17 (Project AFRITIMB).

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Authors and Affiliations

  1. Evolutionary Biology and Ecology, Université Libre de Bruxelles, Brussels, Belgium

    Oriana B. Bhasin, Saskia Sergeant & Olivier J. Hardy

Authors
  1. Oriana B. Bhasin
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  2. Saskia Sergeant
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  3. Olivier J. Hardy
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Contributions

OB and OJH designed the study. OB contributed to collection of plant material. OB and SS performed the laboratory analyses. OB conducted the data analyses. OB and OJH wrote the first draft and all authors contributed to the final version of the manuscript.

Corresponding author

Correspondence to Oriana B. Bhasin.

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The authors have no relevant financial or non-financial interests to disclose.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Bhasin, O.B., Sergeant, S. & Hardy, O.J. Development and characterization of microsatellite markers in the African timber tree species Cylicodiscus gabunensis (Fabaceae). Mol Biol Rep 49, 11209–11214 (2022). https://doi.org/10.1007/s11033-022-07908-x

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