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Genome editing (CRISPR-Cas)-mediated virus resistance in potato (Solanum tuberosum L.)

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Abstract

Plant viruses are the major pathogens that cause heavy yield loss in potato. The important viruses are potato virus X, potato virus Y and potato leaf roll virus around the world. Besides these three viruses, a novel tomato leaf curl New Delhi virus is serious in India. Conventional cum molecular breeding and transgenics approaches have been applied to develop virus resistant potato genotypes. But progress is slow in develo** resistant varieties due to lack of host genes and long breeding process, and biosafety concern with transgenics. Hence, CRISPR-Cas mediated genome editing has emerged as a powerful technology to address these issues. CRISPR-Cas technology has been deployed in potato for several important traits. We highlight here CRISPR-Cas approaches of virus resistance through targeting viral genome (DNA or RNA), host factor gene and multiplexing of target genes simultaneously. Further, advancement in CRISPR-Cas research is presented in the area of DNA-free genome editing, virus-induced genome editing, and base editing. CRISPR-Cas delivery, transformation methods, and challenges in tetraploid potato and possible methods are also discussed.

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Acknowledgement

Authors thank Director, ICAR-CPRI, Shimla and CABin Scheme (ICAR-IASRI, New Delhi) for necessary support.

Funding

The work is funded by the ICAR-CPRI, Shimla, Himachal Pradesh, India.

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Author notes

  1. Jagesh Kumar Tiwari and Jeevalatha A have contributed equally to this work.

Authors and Affiliations

  1. ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, 171001, India

    Jagesh Kumar Tiwari

  2. ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, 673012, India

    Jeevalatha A.

  3. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Road, New Delhi, 110067, India

    Narendra Tuteja

  4. Amity University, Gurgaon, Manesar, Haryana, 122413, India

    S. M. Paul Khurana

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  1. Jagesh Kumar Tiwari
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  4. S. M. Paul Khurana
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JKT and JA wrote the manuscript. SMPK and NT critically edited and improved the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jagesh Kumar Tiwari.

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Tiwari, J.K., A., J., Tuteja, N. et al. Genome editing (CRISPR-Cas)-mediated virus resistance in potato (Solanum tuberosum L.). Mol Biol Rep 49, 12109–12119 (2022). https://doi.org/10.1007/s11033-022-07704-7

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