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Metabolic imprinting induced by a high-sugar diet: effects on microRNA expression and insulin resistance in young rats

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Abstract

Background

Metabolic imprinting describes associations between nutritional experiences of early life and the development of diseases later in life. The goal of this study was to evaluate the metabolic imprinting induced by a high-sugar diet (HSD) and its effects on microRNA (miRNA) expression and insulin resistance (IR) in young rats. We assessed the effects of expression of adipogenic (miR-200c) and metabolic (miR-126a) miRNAs in retroperitoneal white adipose tissue (rWAT) on IR development.

Methods and results

Weaned male Wistar rats (N = 6) were fed a standard chow diet or HSD (68% carbohydrates) for 4-, 8-, or 12-weeks. Serum samples were collected to measure triacylglycerol and VLDL-cholesterol, and we assessed glucometabolic parameters (glucose, insulin, HOMA-IR, and QUICKI). rWAT was collected for microRNA analysis (N = 3). The HSD resulted in body fat accretion and IR after 8-weeks, which resolved by 12-weeks. Moreover, the HSD had a time-dependent effect on miRNA relative expression, downregulating rno-miR-200c-3p at week 8 and rno-miR-126a-3p at week 12.

Conclusions

MiR-200 family dysregulation has been related to IR, and miR-126a downregulation could be associated with the improvement in IR observed after a 12-week HSD feeding period. This is the first time that excessive sugar intake post-weaning has been associated with miRNA production by rWAT with an impact on IR development.

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Availability of data and material (data transparency)

All data generated or analysed during this study are included in this published article.

Code Availability (software application or custom code)

Not applicable.

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Acknowledgements

The authors would like to acknowledge the Centro de Ciência Animal (CCA) from the Universidade Federal de Ouro Preto for supply of the animals used in this study. Additionally, this work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG APQ-02772-15). We would like to thank Editage (www.editage.com) for their English language editing services.

Funding

This work was supported by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG APQ-02772-15).

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Authors and Affiliations

  1. Laboratório de Bioquímica e Biologia Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brasil

    Karina Barbosa de Queiroz PhD., Elísio Alberto Evangelista & Renata Guerra-Sa

  2. Departamento de Alimentos, Programa de Pós-Graduação em Saúde e Nutrição, Escola de Nutrição, Universidade Federal de Ouro Preto, Ouro Preto, Brasil

    Karina Barbosa de Queiroz PhD.

Authors
  1. Karina Barbosa de Queiroz PhD.
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  2. Elísio Alberto Evangelista
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  3. Renata Guerra-Sa
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Karina Barbosa de Queiroz. The first draft of the manuscript was written by Karina Barbosa de Queiroz and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Karina Barbosa de Queiroz PhD..

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All experimental procedures were approved by the Ethics Committee of the Federal University of Ouro Preto for the Care and Use of Laboratory Animals (Protocol 2011/56) and were conducted in accordance with the regulations described in the Committee’s Guiding Principles Manual.

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Barbosa de Queiroz, K., Evangelista, E.A. & Guerra-Sa, R. Metabolic imprinting induced by a high-sugar diet: effects on microRNA expression and insulin resistance in young rats. Mol Biol Rep 49, 8173–8178 (2022). https://doi.org/10.1007/s11033-022-07473-3

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