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Comparative analysis of differentially expressed miRNAs in leaves of three sugarcanes (Saacharum officinarum L.) cultivars during salinity stress

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Abstract

Background

Sugarcane is an important industrial plant cultivated mostlyin the arid and semiarid regions. Due to climate change and anthropogenic activities, the sugarcane fieldsare prone to be damagedas a result of salt deposition. The consequence of such phenomena is turning to become a major thread in sugarcane cultivation. To address this issue, the identification of salinity tolerant cultivars would be a suitable strategy to minimize yield loss in the area. It is well known thatthe expression of abiotic stress-responsive genes including noncoding microRNAs (miRNAs) and their codingtargetscould lead to enhancement of stress tolerance in crops. Therefore, the expression study of those noncoding and coding genes under stress conditions is an appropriate approach to screen the tolerant cultivars. In addition, the examination of the expression of miRNA’s target genes could provide deeper insight into the molecular stress mechanism and facilitate the identification of tolerant cultivars.

Methods and results

We aimedto assess the expression of nine candidate miRNAsand their corresponding targeted genes among the studied sugarcane cultivars under high salinity conditions, leading to the identification of the salt-tolerant cultivar. To achieve our goal, a two-factorial experiment with three sugarcane cultivars (CP-48, CP-57, CP-69) and two salinity levels (0 and 8 ds/m) was conducted. The result indicated significant differences in expression with in miRNAs and also their target genes. The highest reduction of miRNAs expression occurred in miR160 while the lowest oneappeared in miR1432. The data also indicated that the higher and the lowest expression of targeted genes occurred in miR160 and miR393 respectively. Among studied cultivars, the CP-57 showed poor performance while CP-69 expresses a superior tolerance to salt stress.

Conclusions

Taken together, these results suggested that the monitoring of microRNA expressioncould provide a new approach forthe screening of well-adapted cultivars under salt conditions. Such an approach would be the appropriate solutionto combat plant stress inhigh salinity region/soil. Our result indicated that the miR160 generates sugarcane tolerant to salt stress, can be potentially be used as a biomarker to salt stress.

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Acknowledgements

We thank Dr. H. Hamdi the research center chief of sugarcane development, Ahvaz, Iran, for the donation of sugarcanes seeds.

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Authors and Affiliations

  1. Department of Plant Production and Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Tofigh Mazalmazraei, Leila Nejadsadeghi, Khosro Mehdi Khanlou & Daryoosh Nabati Ahmadi

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  1. Tofigh Mazalmazraei
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  2. Leila Nejadsadeghi
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  3. Khosro Mehdi Khanlou
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  4. Daryoosh Nabati Ahmadi
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Contributions

Writing–original draft and investigation:Tofigh Mazalmazraei; Supervision andproject administration: Leila Nejhad sadeghi; Data curation and formal analysis: Khosro Mehdi Khanlou; Validation: Daryoosh Nabati Ahmadi.

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Correspondence to Leila Nejadsadeghi.

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Mazalmazraei, T., Nejadsadeghi, L., Mehdi Khanlou, K. et al. Comparative analysis of differentially expressed miRNAs in leaves of three sugarcanes (Saacharum officinarum L.) cultivars during salinity stress. Mol Biol Rep 50, 485–492 (2023). https://doi.org/10.1007/s11033-022-07349-6

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  • DOI: https://doi.org/10.1007/s11033-022-07349-6

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