Abstract
Background
The intensive application of inorganic nitrogen has contributed to increasing the crop yield with the risk of environmental damage. High nitrogen fertilizer requirements are a long-standing problem in japonica cultivation.
Materials and methods
In the present study, 200 recombinant inbred lines derived from two representative japonica varieties of Japanese and Chinese varieties, Akitakomachi and Liaogeng5, respectively, were planted under different nitrogen fertilization conditions. The relationships among nitrogen fertilization, genetic background, and important agronomic traits were surveyed through the phenotypic investigation and next-generation sequencing.
Results
The results showed that the yield components of Akitakomachi dramatically decreased along with the nitrogen reduction, whereas those of Liaogeng5 exhibited a slight decrease. The appearance quality and milling quality of both Akitakomachi and Liaogeng5 were improved, especially Liaogeng5. The recombinant inbred lines (RILs) showed similar trends to their parental lines. The QTLs analysis of important agronomic traits detected 17 loci, one QTL clustering located on chromosome 9 that corresponded to the plant height, panicle angle, grain number per panicle, harvest index, and taste value was identified to be synonymous with a previously reported gene, DEP1. The function of DEP1 was verified by a knock-out mutant constructed by CRISPR/Cas9. The dep1 mutant exhibited improved grain quality without severe yield penalty under nitrogen reduction conditions.
Conclusion
The results of the present study provide useful information for the improvement of food security by enhancing nitrogen use efficiency.
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Code availability
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Funding
This study was funded by The National Natural Science Foundation of China, Grant Number U1708231.
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QX, ZX, and HX designed this study and contributed to the original concept of the project. GH and SD performed most of the experiments. QX, GH, and HX wrote the paper. All authors read and approved the final manuscript.
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He, G., Du, Z., Xu, Z. et al. Genome sequencing and genetic analysis of recombinant inbred lines reveals important agronomic traits related loci under different nitrogen fertilization. Mol Biol Rep 49, 4545–4553 (2022). https://doi.org/10.1007/s11033-022-07298-0
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DOI: https://doi.org/10.1007/s11033-022-07298-0