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Nonadditive expression of lipid metabolism pathway-related genes in intestine of hybrids of Nile tilapia females (Oreochromis niloticus) and blue tilapia males (Oreochromis aureus)

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Abstract

Nonadditive expression contributes to heterosis in hybrids. In this study, the expression profiles of twelve lipid metabolism pathway-related genes were investigated in the intestine of Nile tilapia (Oreochromis niloticus) ♀ × blue tilapia (Oreochromis aureus) ♂ hybrid. The expression of genes from the hybrid were assigned to nonadditive and additive expression pattern groups and compared with expression patterns from Nile tilapia and blue tilapia. In the intestine of the hybrid, apoA4B was expressed at intermediate levels, but apoB and MTP were assigned to ELD-B and ELD-N categories, respectively. The LPL and LRP1 showed transgressive up-regulation in the hybrid, but LDLR was assigned to the ELD-B category. For fatty acid uptake related genes, only FABP11a was categorized as nonadditive expression with transgressive up-regulation, while CD36 and FABP3 were categorized as additive expression in the intestine of the hybrid. Two genes in triacylglycerol metabolism, namely, FAS and DGAT2, showed transgressive up-regulation in the hybrid. Most of the genes analyzed in the present study showed nonadditive expression (8 in 12), and five genes showed transgressive up-regulation. These results indicated that the stimulation of lipid metabolism in the hybrid compared to that of its parents. The hyperactive expression of these genes in the hybrid may be associated with the growth and lipid usage vigor.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31460688, 31672627, 31760756), the Natural Science Foundation of Guangxi (Grant No. 2017GXNSFFA198001); Public welfare of institute research funding in Guangxi (Grant No. CXIF-2016-14) and China Agriculture Research System (CARS-46).

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Authors and Affiliations

  1. Guangxi Tilapia Genetic Breeding Center, Guangxi Academy of Fishery Sciences, Nanning, 530021, China

    Yi Zhou, **ao** Zhang, Jun **ao, Zhongbao Guo, Yongju Luo & Huan Zhong

  2. Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    **ao** Zhang & Yongju Luo

  3. Department of Cell Biology, School of Life Sciences, Central South University, Changsha, 410017, China

    Qian Xu & **peng Yan

  4. Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Fan Yu

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  1. Yi Zhou
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Correspondence to Huan Zhong.

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The authors declared that they have no conflicts of interest. The funding agencies had no influence on the experimental design and the conclusions.

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All animal handling complied with the requirements of the Animal Research and Ethics Committees of Guangxi Academy of Fishery Sciences.

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Zhou, Y., Zhang, X., Xu, Q. et al. Nonadditive expression of lipid metabolism pathway-related genes in intestine of hybrids of Nile tilapia females (Oreochromis niloticus) and blue tilapia males (Oreochromis aureus). Mol Biol Rep 46, 425–432 (2019). https://doi.org/10.1007/s11033-018-4490-3

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  • DOI: https://doi.org/10.1007/s11033-018-4490-3

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