Abstract
Myostatin (MSTN) is a negative regulator of skeletal muscle mass and has a potential application in aquaculture. We reported the characterization of the myostatin gene and its expression in the croceine croaker, Pseudosciaena crocea. The myostatin gene had three exons encoding 376 amino acids. The cDNA was 1,906 bp long with a 5′-UTR and 3′-UTR of 108 bp and 667 bp, respectively. A microsatellite sequence, CA30 and CA26 separated by TA, existed in the 3′-UTR. Intron I and II were 343 bp and 758 bp in length, respectively. The deduced amino acid sequence was highly conserved, and had more than 90% identical to shi drum, gilthead seabream, striped sea-bass, white perch, and white bass proteins. The myostatin of croceine croaker had a putative amino terminal signal sequence (residues 1–22), a transforming growth factor-beta (TGF-β) propeptide domain (residues 41–256), a RXXR proteolytic processing site (RARR, residues 264–267, matching the RXXR consensus site), and a TGF-β domain (residues 282–376). There were 13 conserved cysteine residues in croceine croaker myostatin, nine of which are common to all TGF-β superfamily members. The most conserved region of vertebrate myostatins is the TGF-β domain, which was the mature bioactive domain of the myostatin protein. The myostatin gene was expressed not only in the skeletal muscle, but also in the other tissues.
Similar content being viewed by others
References
AC McPherron AM Lawler SJ Lee (1997) ArticleTitleRegulation of skeletal muscle mass in mice by a new TGF-β superfamily member Nature 387 IssueID6628 83–90 Occurrence Handle9139826 Occurrence Handle1:CAS:528:DyaK2s**vFygur0%3D Occurrence Handle10.1038/387083a0
BD Rodgers GM Weber (2001) ArticleTitleSequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana Comp Biochem Physiol B Biochem Mol Biol 129 IssueID2–3 597–603 Occurrence Handle11399495 Occurrence Handle1:STN:280:DC%2BD3MzjtFKqsg%3D%3D Occurrence Handle10.1016/S1096-4959(01)00350-5
J Lin HB Arnold MA Della-Fera MJ Azain DL Hartzell CA Baile (2002) ArticleTitleMyostatin knockout in mice increases myogenesis and decreases adipogenesis Biochem Biophys Res Commun 291 701–706 Occurrence Handle11855847 Occurrence Handle1:CAS:528:DC%2BD38XhtlGmuro%3D Occurrence Handle10.1006/bbrc.2002.6500
AC McPherron SJ Lee (1997) ArticleTitleDouble muscling in cattle due to mutations in the myostatin gene Proc Natl Acad Sci 94 IssueID23 12457–12461 Occurrence Handle9356471 Occurrence Handle1:CAS:528:DyaK2sXns1ylsbk%3D Occurrence Handle10.1073/pnas.94.23.12457
R Kambadur M Sharma TP Simth JJ Bass (1997) ArticleTitleMutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle Genome Res 7 910–916 Occurrence Handle9314496 Occurrence Handle1:CAS:528:DyaK2sXmtVSlu7w%3D
C Xu G Wu Y Zohar SJ Du (2003) ArticleTitleAnalysis of myostatin gene structure, expression and function in zebrafish J Exp Biol 206 4067–4079 Occurrence Handle14555747 Occurrence Handle1:CAS:528:DC%2BD2cXhvFGqsA%3D%3D Occurrence Handle10.1242/jeb.00635
J Acosta Y Carpio I Borroto O Gonzalez MP Estrada (2005) ArticleTitleMyostatin gene silenced by RNAi show a zebrafish giant phenotype J Biotechnol 119 IssueID4 324–331 Occurrence Handle16005092 Occurrence Handle1:CAS:528:DC%2BD2MXhtVarsLnL Occurrence Handle10.1016/j.jbiotec.2005.04.023
L Maccatrozzo L Bargelloni P Patarnello G Radaelli F Mascarello T Patarnello (2002) ArticleTitleCharacterization of the myostatin gene and a linked microsatellite marker in shi drum (Umbrina cirrosa, Sciaenidae) Aquaculture 205 IssueID1–2 49–60 Occurrence Handle1:CAS:528:DC%2BD38XovFehtw%3D%3D Occurrence Handle10.1016/S0044-8486(01)00659-7
PR Biga KD Cain RW Hardy GT Schelling K Overturf SB Roberts FW Goetz TL Ott (2004) ArticleTitleThe isolation, characterization, and expression of a novel GDF11 gene and a second myostatin form in zebrafish, Danio rerio Gener Comp Endocrinol 138 IssueID1 32–41 Occurrence Handle1:CAS:528:DC%2BD2cXlsFynsLs%3D Occurrence Handle10.1016/j.ygcen.2004.05.001
AM Kocabas H Kucuktas RA Dunham Z Liu (2002) ArticleTitleMolecular characterization and differential expression of the myostatin gene in channel catfish (Ictalurus punctatus) Biochem Biophysiol Acta-Gene Struc Express 1575 IssueID1–3 99–107 Occurrence Handle1:CAS:528:DC%2BD38XjvVaqu78%3D
J Sambrook EF Fritsch T Maniatis (1989) Molecular cloning: a laboratory manual EditionNumber2 Cold Spring Harbor Laboratory Press Cold Spring Harbor
SF Altschul TL Madden AA Sch+þffr J Zhang Z Zhang W Miller DJ Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucl Acids Res 25 3389–3402 Occurrence Handle9254694 Occurrence Handle1:CAS:528:DyaK2sXlvFyhu7w%3D Occurrence Handle10.1093/nar/25.17.3389
JD Thompson DG Higgins TJ Gibson (1994) ArticleTitleClustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalities and weight matrix choice Nucl Acids Res 22 4673–4680 Occurrence Handle7984417 Occurrence Handle1:CAS:528:DyaK2M**tlSgu74%3D
S Kumar K Tamura M Nei (2004) ArticleTitleMEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment Briefings Bioinformat 5 150–163 Occurrence Handle1:CAS:528:DC%2BD2cXntFGqu7s%3D Occurrence Handle10.1093/bib/5.2.150
L Maccatrozzo L Bargelloni G Radaelli F Mascarello T Patarnello (2001) ArticleTitleCharacterization of the myostatin gene in the gilthead seabream (Sparus aurata): sequence, genomic structure, and expression pattern Mar Biotechnol 3 224–230 Occurrence Handle14961359 Occurrence Handle1:CAS:528:DC%2BD3MXkvFClsb8%3D Occurrence Handle10.1007/s101260000064
RS Thies T Chen MV Davies KN Tomkinson AA Pearson QA Shakey NM Wolfman (2001) ArticleTitleGDF-8propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor binding Growth Factors 18 251–259 Occurrence Handle11519824 Occurrence Handle1:STN:280:DC%2BD3Mvns1Oltw%3D%3D Occurrence Handle10.3109/08977190109029114
SJ Lee AC McPherron (2001) ArticleTitleRegulation of myostatin activity and muscle growth Proc Natl Acad Sci USA 98 9306–9311 Occurrence Handle11459935 Occurrence Handle1:CAS:528:DC%2BD3MXlvFSrsL0%3D Occurrence Handle10.1073/pnas.151270098
L Grobet LJ Martin D Poncelet D Pirottin B Brouwers J Riquet A Schoeberlein S Dunner F Menissier J Massabanda R Fries R Hanset M Georges (1997) ArticleTitleA deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle Nat Genet 17 71–74 Occurrence Handle9288100 Occurrence Handle1:CAS:528:DyaK2sXlvVGhtr8%3D Occurrence Handle10.1038/ng0997-71
P Wiener JA Smith AM Lewis JA Woolliams JL Williams (2002) ArticleTitleMuscle-related traits in cattle: the role of the myostatin gene in the South Devon breed Genet Sel Evol 34 221–232 Occurrence Handle12081809 Occurrence Handle1:CAS:528:DC%2BD38Xmt1antrw%3D Occurrence Handle10.1051/gse:2002005
M Sharma R Kambadur KG Matthews WG Somers GP Devlin JV Conaglen PJ Fowke JJ Bass (1999) ArticleTitleMyostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct J Cell Physiol 180 1–9 Occurrence Handle10362012 Occurrence Handle1:CAS:528:DyaK1MXjs1Cgs7Y%3D Occurrence Handle10.1002/(SICI)1097-4652(199907)180:1<1::AID-JCP1>3.0.CO;2-V
S Ji RL Losinski RL Cornelius SG Frank GM Willis DE Gerrard FF Depreux ME Spurlock (1998) ArticleTitleMyostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation Am J Physiol 275 1265–1273
BD Rodgers GM Weber CV Sullivan MA Levine (2001) ArticleTitleIsolation and characterization of myostatincomplementary deoxyribonucleic acid clones from two commercially important fish: Oreochromis mossambicus and Morone chrysop Endocrinology 142 1412–1418 Occurrence Handle11250920 Occurrence Handle1:CAS:528:DC%2BD3M**tlemtLc%3D Occurrence Handle10.1210/en.142.4.1412
SB Roberts FW Goetz (2001) ArticleTitleDifferential skeletal muscle expression of my across teleost species, and the isolation of multiple myostatin isoform FEBS Lett 491 212–216 Occurrence Handle11240129 Occurrence Handle1:CAS:528:DC%2BD3MXhs1ehsrs%3D Occurrence Handle10.1016/S0014-5793(01)02196-2
L Maccatrozzo L Bargelloni B Cardazzo G Rizzo T Patarnello (2001) ArticleTitleA novel second myostatin gene is present in teleost fish FEBS Lett 509 36–40 Occurrence Handle11734202 Occurrence Handle1:CAS:528:DC%2BD3MXovVCisb0%3D Occurrence Handle10.1016/S0014-5793(01)03124-6
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xue, L., Qian, K., Qian, H. et al. Molecular Cloning and Characterization of the Myostatin Gene in Croceine Croaker, Pseudosciaena crocea . Mol Biol Rep 33, 129–135 (2006). https://doi.org/10.1007/s11033-006-0015-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11033-006-0015-6