Abstract
European winter wheat cultivar “Tabasco” was reported to have resistance to powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) in China. In previous studies, Tabasco was reported to have the resistance gene designated as Pm48 on the short arm of chromosome 5D when a map** population was phenotyped with pathogen isolate Bgt19 collected in China and was genotyped with simple sequence repeat (SSR) markers. In this study, single-nucleotide polymorphism (SNP) chips were used to rapidly determine the resistance gene by map** a new F2 population that was developed from Tabasco and a susceptible cultivar “Ningmaizi119” and inoculated with pathogen isolate NCF-D-1–1 that was collected in the USA. The segregation of resistance in the population was found to link with Pm2 which was identified in Tabasco. Therefore, it was concluded that the previously reported Pm48 on chromosome arm 5DS in Tabasco should be the Pm2 gene on the same chromosome. The Pm2 was also found in European cultivars “Mattis” and “Claire” but not in any of the accessions from diploid wheat Aegilops tauschii or modern cultivars such as “Gallagher,” “Smith’s Gold,” and “OK Corral” being used in the Great Plains in the USA. A KASP marker was developed to track the resistance allele Pm2 in wheat breeding.
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Acknowledgements
We thank Dr. Bob Hunger at Oklahoma State University for providing assistance in phenoty**. We also thank Dr. Christina Cowger at the USDA-ARS at Raleigh for providing isolate NCF-D-1-1.
Funding
This study was partially funded by the National Natural Science Foundation of China (No. 31971889) and the International Cooperation Fund of Jiangsu Academy of Agricultural Sciences. This project was supported by Agriculture and Food Research Initiative Competitive Grants (2017–67007-25939 and 2022–68013-36439) from the USDA National Institute of Food and Agriculture (NIFA).
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Jizhong Wu and Haiyan Jia performed most of the phenoty** and molecular experiments; Linyi Qiao and Bisheng Fu undertook part of the phenoty** work; Ragupathi Nagarajan developed KASP marker; Gina Brown-Guedira screened parental lines; Liuling Yan directed research work; Jizhong Wu, Haiyan Jia, and Liuling Yan wrote the manuscript.
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Wu, J., Jia, H., Qiao, L. et al. Genetic basis of resistance against powdery mildew in the wheat cultivar “Tabasco”. Mol Breeding 43, 56 (2023). https://doi.org/10.1007/s11032-023-01402-3
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DOI: https://doi.org/10.1007/s11032-023-01402-3