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Genome-wide association map** of resistance to the foliar diseases septoria nodorum blotch and tan spot in a global winter wheat collection

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Abstract

Septoria nodorum blotch (SNB) and tan spot, caused by the necrotrophic fungal pathogens Parastagonospora nodorum and Pyrenophora tritici-repentis, respectively, often occur together as a leaf spotting disease complex on wheat (Triticum aestivum L.). Both pathogens produce necrotrophic effectors (NEs) that contribute to the development of disease. Here, genome-wide association analysis of a diverse panel of 264 winter wheat lines revealed novel loci on chromosomes 5A and 5B associated with sensitivity to the NEs SnTox3 and SnTox5 in addition to the known sensitivity genes for NEs Ptr/SnToxA, SnTox1, SnTox3, and SnTox5. Sensitivity loci for SnTox267 and Ptr ToxB were not detected. Evaluation of the panel with five P. nodorum isolates for SNB development indicated the Snn3-SnTox3 and Tsn1-SnToxA interactions played significant roles in disease development along with additional QTL on chromosomes 2A and 2D, which may correspond to the Snn7-SnTox267 interaction. For tan spot, the Tsc1-Ptr ToxC interaction was associated with disease caused by two isolates, and a novel QTL on chromosome 7D was associated with a third isolate. The Tsn1-ToxA interaction was associated with SNB but not tan spot. Therefore some, but not all, of the previously characterized host gene-NE interactions in these pathosystems play significant roles in disease development in winter wheat. Based on these results, breeders should prioritize the selection of resistance alleles at the Tsc1, Tsn1, Snn3, and Snn7 loci as well as the 2A and 7D QTL to obtain good levels of resistance to SNB and tan spot in winter wheat.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We would like to thank Danielle Holmes, Cayley Steen, Erika Shay Bauer, Molly Holt, Jonathan Schwartz, Brandon Rasmusson, Lydia Lyons, Megan Overlander, and Stephanie McCoy for their help with greenhouse work and phenoty**. We would like to thank Gayan Kariyawasam and Jonathan Richards for their assistance with fungal isolates. We would like to thank Nathan Wyatt and Jason Fiedler for assistance with GWAS analysis. Lastly, we would like to thank Mike Pumphrey for providing seed for this experiment. This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the author’s and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

Funding

This research was supported by USDA-ARS CRIS 3060–21000-038-00D.

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Authors and Affiliations

  1. Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, , Fargo, ND, 58102, USA

    Amanda R. Peters Haugrud, Zengcui Zhang, Agnes Szabo-Hever, Timothy L. Friesen & Justin D. Faris

  2. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58102, USA

    Gongjun Shi & Zhaohui Liu

  3. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58102, USA

    Sudeshi Seneviratne, Katherine L. D. Running, Gurminder Singh & Krishna Acharya

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  1. Amanda R. Peters Haugrud
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Contributions

ARPH, TLF, ZL, and JDF conceived and designed the experiments. ARPH, GS, SS, KLDR, ZZ, GS, ASH, KA, and ZL performed the experiments. ZL and TLF contributed materials. ARPH analyzed the data. The first draft of the manuscript was written by ARPH and JDF and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Justin D. Faris.

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Peters Haugrud, A.R., Shi, G., Seneviratne, S. et al. Genome-wide association map** of resistance to the foliar diseases septoria nodorum blotch and tan spot in a global winter wheat collection. Mol Breeding 43, 54 (2023). https://doi.org/10.1007/s11032-023-01400-5

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