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Positional map** and identification of novel quantitative trait locus responsible for UV-B radiation tolerance in soybean [Glycine max (L.) Merr.]

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Abstract

The amount of ultraviolet-B radiation (UV-B: 280–320 nm) reaching Earth’s surface is expected to increase due to stratospheric ozone depletion. This could cause significant biological damage in plants, and serious yield losses in crops. Soybean [Glycine max (L.) Merr.], a major legume crop, is known to be sensitive to UV-B radiation. Thus, develo** a UV-B-tolerant soybean is an efficient and economical strategy to avoid putative yield losses through increased UV-B irradiation. The objective of this study is to identify the novel quantitative trait loci (QTLs) for UV-B tolerance in the soybean using high-density genetic linkage map**. One hundred and fifteen F8-derived F12 recombinant inbred lines developed from a cross between the UV-B susceptible cultivar, Keunol, and a tolerant breeding line, Iksan 10, were used. Three categories of phenotypic traits were scored: degree of leaf color change, degree of leaf shape change and degree of total plant damage. A genome-wide molecular genetic linkage map containing 8691 single nucleotide polymorphism markers was constructed using the recently developed genoty** platform, the 180K Axiom SoyaSNP assay. Using composite interval map** analysis, one major candidate QTL on chromosome 7 was identified and designated qUVBT1, and is located between two flanking makers, AX-90437826 and AX-90317546, within 1.6 cM, corresponding to a ~24-kb physical region with six annotated gene models. One of them is a homolog of yeast RAD23, which has previously been reported to be a UV excision repair protein. This result could be valuable in breeding new UV-B-tolerant soybean cultivars and elucidating the UV-B response mechanism in soybean plants.

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Abbreviations

QTL:

Quantitative trait locus

SNP:

Single nucleotide polymorphism

RIL:

Recombinant inbred line

DTP:

Degree of total plant damage

DLC:

Degree of leaf color chlorosis

DLS:

Degree of leaf shape change

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Acknowledgments

This work was carried out with the support of National Research Foundation of Korea (Project No. NRF-2013R1A1A2009812).

Author contribution

JSL designed and conducted field tests and drafted the manuscript. SK conducted field tests. BKH helped to construct genetic linkage map and QTL analysis. STK designed the experiment and organized the manuscript. All authors read and approved the final manuscript. Authors state that the experiments comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. Department of Crop Science and Biotechnology, Dankook University, Cheonan, 330-714, Korea

    Ju Seok Lee, Sungmin Kim & Sungtaeg Kang

  2. Department of Plant Biotechnology, Chonnam National University, Gwangju, 500-757, Korea

    Bo-Keun Ha

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  1. Ju Seok Lee
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  2. Sungmin Kim
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Correspondence to Sungtaeg Kang.

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Lee, J.S., Kim, S., Ha, BK. et al. Positional map** and identification of novel quantitative trait locus responsible for UV-B radiation tolerance in soybean [Glycine max (L.) Merr.]. Mol Breeding 36, 50 (2016). https://doi.org/10.1007/s11032-016-0471-1

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