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Structural analysis of the complex of phenoxazone antibiotic actinocyl-bis(2-dimethylaminoethyl)amide with deoxytetranucleotide 5′-d(TpGpCpA) by two-dimensional 1H-NMR spectroscopy and molecular mechanics

  • Structural and Functional Analysis of Biopolymers and their Complexes
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Abstract

The spatial structure of an intercalated complex of the synthetic phenoxazone antibiotic actinocyl-bis-(2-dimethylaminoethyl)amide (ActII) and the self-complementary deoxytetranucleotide 5′-d(TpGpCpA) was studied using two-dimensional NMR (2D-NOESY) spectroscopy of the complex in an aqueous solution and molecular mechanics simulation. Distinctive features of the conformation of ActII-DNA complexes were determined for two possible orientations of the ActII chromophore at the intercalation site.

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Authors and Affiliations

  1. Department of Physics, Sevastopol National Technical University, Sevastopol, 99053, Ukraine

    A. N. Veselkov, L. N. Djimant & K. A. Veselkov

  2. School of Biological and Chemical Sciences, Birkbeck College, University of London, London WCIE, 7HX, United Kingdom

    D. B. Davies

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  1. A. N. Veselkov
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  2. L. N. Djimant
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  3. K. A. Veselkov
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  4. D. B. Davies
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__________

Translated from Molekulyarnaya Biologiya, Vol. 39, No. 2, 2005, pp. 336–344.

Original Russian Text Copyright © 2005 by A. Veselkov, Djimant, K. Veselkov, Davies.

Deceased.

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Veselkov, A.N., Djimant, L.N., Veselkov, K.A. et al. Structural analysis of the complex of phenoxazone antibiotic actinocyl-bis(2-dimethylaminoethyl)amide with deoxytetranucleotide 5′-d(TpGpCpA) by two-dimensional 1H-NMR spectroscopy and molecular mechanics. Mol Biol 39, 298–305 (2005). https://doi.org/10.1007/s11008-005-0042-8

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  • DOI: https://doi.org/10.1007/s11008-005-0042-8

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