SpringerLink
Log in

Anti-bacterial effect of four extracts from leaves of Dracontomelon dao on Escherichia coli growth using microcalorimetry coupled with principal component analysis

A reliable evaluation model of quantity–anti-bacterial effect relationship

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

The anti-Escherichia coli activities of four extracts in leaves of Dracontomelon dao, a traditional folk herb in China were investigated and compared by microcalorimetry. The four extracts are PE fraction, CHCl3 fraction, EtOAc fraction, and n-BuOH fraction. The heat flow power–time (HFP–time) curves of E. coli growth in the presence of the four extracts were measured using an ampoule method. Then the nine thermal kinetic parameters were obtained from the curves. From the result of principal component analysis, it can be seen that parameters k 1, k 2, P 1, and Q p2 might be the main parameters in evaluating the anti-E. coli effects. In the presence of CHCl3 fraction, EtOAc fraction, and n-BuOH fraction, k 2, Q p2 of E. coli decreased with increasing concentrations of the extracts. The EtOAc fraction was observed to have the strongest anti-bacterial activity with half-inhibitory concentration IC50 of 98.5 μg mL−1. So, it can be concluded that EtOAc fraction can be further developed as anti-bacterial bioactive fraction of leaves of Dracontomelon dao.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Thailand)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Khan MR, Omoloso AD. Antibacterial and antifungal activities of Dracontomelon dao. Fitoterapia. 2002;73:327–30.

    Article  CAS  Google Scholar 

  2. Su XF, Nong WT. Study on the antimicrobial effect of the extracts of leaves of Dracontomelon dao. China Dispens. 2010;21:2115–7.

    Google Scholar 

  3. Su XF, Liang ZY, Nong KL. Study on chemical constituents of volatile oil of leaves of Dracontomelon dao. Chin Pat Drug. 2008;30:1549–50.

    CAS  Google Scholar 

  4. Wadsö I. Isothermal microcalorimety: current problems and prospects. J Therm Anal Calorim. 2001;64:75–84.

    Article  Google Scholar 

  5. Kong WJ, Zhao YL, Shan LM, **ao XH, Guo WY. Investigation on the spectrum–effect relationships of EtOAc extract from Radix Isatidis based on HPLC fingerprints and microcalorimetry. J Chromatogr B. 2008;871:109–14.

    Article  CAS  Google Scholar 

  6. Li X, Liu Y, Jun W, Liang HG, Qu SS. Microcalorimetric study of Staphylococcus aureus growth affected by selenium compounds. Thermochim Acta. 2002;387:57–61.

    Article  CAS  Google Scholar 

  7. Yao J, Tian L, Wang F, Chen HL, Xu CQ, Su CL, Cai MF, Maskow T, Zaray G, Wang YX. Microcalorimetric study on effect of chromium (III) and chromium (VI) species on the growth of Escherichia coli. Chin J Chem. 2008;26:101–6.

    Article  CAS  Google Scholar 

  8. McGulnness MS, Barisas BG. Acute toxicity measurements on aquatic pollutants using microcalorimetry on tissue-cultured cells. Environ Sci Technol. 1991;25:921.

    Article  Google Scholar 

  9. Kong WJ, Zhao YL, Shan LM, **ao XH, Guo WY. Thermochemical studies on the quantity–antibacterial effect relationship of four organic acids from Radix Isatidis on Escherichia coli growth. Biol Pharm Bull. 2008;31:1301–5.

    Article  CAS  Google Scholar 

  10. Furustrand Tafin U, Clauss M, Hauser PM, Bille J, Meis JF, Trampuz A. Isothermal microcalorimetry: a novel method for real-time determination of antifungal susceptibility of Aspergillus species. Clin Microbiol Infect. 2012;18:E241–5.

    Article  CAS  Google Scholar 

  11. **e CL, Tang HK, Song ZH, Qu SS, Liao YT, Liu HS. Microcalorimetric study of bacterial growth. Thermochim Acta. 1988;123:33–41.

    Article  Google Scholar 

  12. Chen J, Wang F, Liu J, Lee FS, Wang X, Yang H. Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Anal Chim Acta. 2008;613:184–95.

    Article  CAS  Google Scholar 

  13. Chen Y, Zhu SB, **e MY, Nie SP, Liu W, Li C, Gong XF, Wang YX. Quality control and original discrimination of Ganoderma lucidum based on high-performance liquid chromatographic fingerprints and combined chemometrics methods. Anal Chim Acta. 2008;623:146–56.

    Article  CAS  Google Scholar 

  14. Yi LZ, Yuan DL, Liang YZ, **e PS, Zhao Y. Quality control and discrimination of pericarpium citri reticulatae and pericarpium citri reticulatae viride based on high-performance liquid chromatographic fingerprints and multivariate statistical analysis. Anal Chim Acta. 2007;588:207–15.

    Article  CAS  Google Scholar 

  15. Yi ZB, Yan Y, Liang YZ, Bao Z. Evaluation of the antimicrobial mode of berberine by LC/ESI-MS combined with principal component analysis. J Pharm Biomed Anal. 2007;44:301–4.

    Article  CAS  Google Scholar 

  16. Kong WJ, ** C, **ao XH, Zhao YL, Li ZL, Zhang P, Liu W, Li XF. Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods. J Hazard Mater. 2010;179:742–7.

    Article  CAS  Google Scholar 

  17. Kong WJ, Wang JB, **ng XY, ** C, **ao XH, Zhao YL, Zhang P, Zang QC, Li ZL. Screening for novel antibacterial agents based on the activities of compounds on metabolism of Escherichia coli: a microcalorimetric study. J Hazard Mater. 2011;185:346–52.

    Article  CAS  Google Scholar 

  18. Kong WJ, Zhao YL, **ao XH, ** C, Liu Y, Li ZL. Comparison of anti-bacterial activity of four kinds of alkaloids in Rhizoma Coptidis based on microcalorimetry. Chin J Chem. 2009;27:1186–90.

    Article  CAS  Google Scholar 

  19. Kong WJ, **ao XH, **ao XH, Zhao YL, Wei JH, Wang JB, Yang RC, Yang MH. Effect of berberine on Escherichia coli, Bacillus subtilis, and their mixtures as determined by isothermal microcalorimetry. Appl Microbiol Biotechnol. 2012. doi:10.1007/s00253-012-4302-y.

    Google Scholar 

Download references

Acknowledgements

The authors are grateful for the support from the National Natural Science Foundation of China (81173571; 81303120); the “twelfth Five-Year Plan” Foundation of China people’s Liberation Army (No: CWS11C164) and the major projects of the National Science and Technology (2012ZX10005010-002-002).

Author information

Authors and Affiliations

  1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China

    Shuxian Liu, Nan Zeng & Tiantian Liu

  2. China Military Institute of Chinese Medicine, 302 Hospital of People’s Liberation Army, 100, The 4th Ring Road, Bei**g, 100039, China

    Shuxian Liu, Yanling Zhao, Tiantian Liu, Yaming Zhang & **aohe **ao

  3. Department of Pharmacy, Guang Dong Pharmacy College, 68, Nanhua District, Guangzhou, 51006, People’s Republic of China

    Bin Han

  4. Department of Integrative Medical Center, 302 Hospital of People’s Liberation Army, 100, The 4th Ring Road, Bei**g, 100039, People’s Republic of China

    Jianyu Li, Lifu Wang, Ruilin Wang, Man Gong & Yonggang Li

Authors
  1. Shuxian Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanling Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nan Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tiantian Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yaming Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bin Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jianyu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lifu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ruilin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Man Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Yonggang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. **aohe **ao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yanling Zhao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, S., Zhao, Y., Zeng, N. et al. Anti-bacterial effect of four extracts from leaves of Dracontomelon dao on Escherichia coli growth using microcalorimetry coupled with principal component analysis. J Therm Anal Calorim 116, 491–497 (2014). https://doi.org/10.1007/s10973-013-3516-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-013-3516-2

Keywords

Search

Navigation