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A drug delivery system with fluorescent labeling for the delivery and release of the carboxylate-type camptothecin and its cytotoxicity on HepG2 cancer cell

  • Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications
  • Published:
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Abstract

To facilitate the loading and targeted delivery of drugs, the camptothecin with negatively charged is used for the intercalation of Eu3+-doped MgAl-LDH with fluorescent property. X-ray diffraction (XRD) results show that the basal spacing (27.3 Å) of the intercalated sample is significantly greater than that of the initial Eu3+-doped MgAl-LDH (8.6 Å), implying the intercalation of the carboxylate-type camptothecin in the interlayer of the Eu3+-doped MgAl-LDH. The in vitro release assay in phosphate buffer solution (PBS) at pH of 7.4 reveals that the camptothecin shows a sustained release and the release is up to 900 min. The cumulative concentration (C) of the releasing camptothecin versus time (t) is fitted by the ExpDec2 Model, and is consistent with the equation: C=A1exp(-t/t1)+A2exp(-t/t2) + C0 (R2 = 0.99886). In addition, the fluorescent signals of the drug delivery system differ significantly during delivery and release of camptothecin, by which the delivery and release of camptothecin may be “recognized”. Cytotoxicity assay confirms that the Eu3+-doped MgAl-LDH loaded with the carboxylate-type camptothecin takes an obvious anticancer effect against HepG2 cancer cell, and the anticancer effect is better than that of the pure lactone-type camptothecin, which is very different from the prevailing view that the carboxylate camptothecin has no anticancer activity.

Graphical Abstract

Highlights

  • The intercalation of Camp into Eu3+-doped MgAl-LDH.

  • Drug delivery system exhibited different fluorescence during drug delivery and release.

  • The carboxylate-type Camp intercalated in the Eu3+-doped LDH exhibited obvious anticancer effect.

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  1. College of Chemistry and Chemical Engineering, Nanchang University, Jiangxi province, Nanchang, 330031, PR China

    Yijun Liu, Tingting Li, **ngxing Qiu, **aoqiang Shang & Yufeng Chen

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Liu, Y., Li, T., Qiu, X. et al. A drug delivery system with fluorescent labeling for the delivery and release of the carboxylate-type camptothecin and its cytotoxicity on HepG2 cancer cell. J Sol-Gel Sci Technol 108, 640–654 (2023). https://doi.org/10.1007/s10971-023-06218-3

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