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Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells

  • Brief Communication: Sol-gel, hybrids and solution chemistries
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Abstract

Lipid-coated mesoporous silica nanoparticles (LC-MSNs) have recently emerged as a next-generation cargo delivery nanosystem combining the unique attributes of both the organic and inorganic components. The high surface area biodegradable inorganic mesoporous silica core can accommodate multiple classes of bio-relevant cargos in large amounts, while the supported lipid bilayer coating retains the cargo and increases the stability of the nanocarrier in bio-relevant media which should promote greater bio-accumulation of LC-MSNs in cancer sites. In this contribution, we report on the optimization of various sol–gel synthesis (pH, stirring speed) and post-synthesis (hydrothermal treatment) procedures to enlarge the MSN pore size and tune the surface chemistry so as to enable loading and delivery of large biomolecules. The proof of concept of the dual cargo-loaded nanocarrier has been demonstrated in immortalized cervical cancer HeLa cells using MSNs of various fine-tuned pore sizes.

Highlights

  • Lipid-coated mesoporous silica nanoparticles were prepared for dual cargo delivery to cancer cells.

  • The pore and particle sizes, surface areas, and condensation degrees were tuned by sol–gel processes.

  • Sol–gel (pH, stirring speed) and post-synthesis (hydrothermal treatment) parameters were optimized.

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Acknowledgements

This work was supported by the Sandia National Laboratories' Laboratory Directed Research and Development (LDRD) program and the Lymphoma and Leukemia Society (LLS) (A.N., E.A.H., J.G.C., P.N.D., J.O.A. and C.J.B.). Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-NA0003525. This paper describes objective technical results and analysis. Any subjective views or opinions that might be expressed in the paper do not necessarily represent the views of the U.S. Department of Energy or the United States Government.

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  1. These authors contributed equally: Achraf Noureddine, Elizabeth A. Hjelvik.

Authors and Affiliations

  1. Chemical and Biological Engineering, University of New Mexico, 210 University Blvd NE, Albuquerque, NM, 87131-0001, USA

    Achraf Noureddine, Elizabeth A. Hjelvik, Jonas G. Croissant, Paul N. Durfee, Jacob O. Agola & C. Jeffrey Brinker

  2. Center for Micro-Engineered Materials, Advanced Materials Laboratory, University of New Mexico, MSC04 2790, 1001 University Blvd SE, Suite 103, Albuquerque, NM, 87106, USA

    C. Jeffrey Brinker

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  1. Achraf Noureddine
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  2. Elizabeth A. Hjelvik
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  3. Jonas G. Croissant
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  5. Jacob O. Agola
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Correspondence to C. Jeffrey Brinker.

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Noureddine, A., Hjelvik, E.A., Croissant, J.G. et al. Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells. J Sol-Gel Sci Technol 89, 78–90 (2019). https://doi.org/10.1007/s10971-018-4772-1

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