SpringerLink
Log in

Novel radioassay for anti-thyroperoxidase autoantibodies using protein A coupled magnetizable cellulose particles as an immunoadsorbent

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Autoantibodies against thyroid peroxidase (TPO); described initially as thyroid microsomal antigen, are found in most of the patients with autoimmune thyroid disorders. The present study comprised of development, optimization and validation of ‘sandwich’ radioassay for the measurement of TPOAb in human serum. The standardized TPOAb radioassay has maximum binding of 25–30%, non-specific binding of < 0.4%, analytical sensitivity of approximately around 1 IU/ml, acceptable intra- and inter-assay %CV, with a standard working range up to 1400 IU/ml. The in-house developed assay is simple, robust and cost-effective, and therefore can be used for routine measurement of TPOAb in clinical samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. D’Aurizio F, Metus P, Polizzi AA, Villalta D, Ferrari A, Castello R, Giani G, Tonutti E, Bizzaro N, Tozzoli R (2015) Autoimmun Highlights 6(3):31–37

    Article  Google Scholar 

  2. Sinclair D (2008) Autoimmunity 41(1):46–54

    Article  CAS  Google Scholar 

  3. Gholve CS, Kumarasamy J, Kulkarni S, Rajan MGR (2017) Ind J Clin Biochem 32(1):39

    Article  CAS  Google Scholar 

  4. Ojo OA, Ikem RT, Kolawole BA, Ojo OE, Ajala MO (2019) JEMDSA 24(3):92–97

    Google Scholar 

  5. Gholve CS, Kumarasamy J, Damle A, Kulkarni S, Rajan MGR (2016) Indian J Appl Res 6(3):468–472

    Google Scholar 

  6. Gholve CS, Kumarasamy J, Damle A, Kulkarni S, Venkatesh M, Banerjee S, Rajan MGR (2018) Ind J Clin Biochem. https://doi.org/10.1007/s12291-018-0768-4

    Article  Google Scholar 

  7. Czarnocka B, Eschler DC, Godlewska M, Tomer Y (2013) Autoantibodies, 3rd edn. Elsevier BV, Amsterdam, pp 365–373

    Google Scholar 

  8. Greenwood FC, Hunter WM, Klopper A (1964) Br Med J 1(5374):22–24

    Article  CAS  Google Scholar 

  9. Fraker PJ, Speck JC (1978) Biochem Biophys Res Commun 80:849–857

    Article  CAS  Google Scholar 

  10. Chard T (1982) An introduction to radioimmunoassay and related techniques, 2nd edn. Elsevier Biomedical Press, Amsterdam

    Google Scholar 

  11. Estienne V, Duthoit C, Costanzo VD, Lejeune PJ, Rotondi M, Kornfeld S, Finke R, Lazarus JH, Feldt-Rasmussen U, Franke WG, Smyth P, D’Herbomez M, Conte-Devolx B, Persani L, Carella C, Jourdain JR, Izembart M, Toubert ME, Pinchera A, Weetman A, Sapin R, Carayon P, Ruf J (1999) Eur J Endocrinol 141:563–569

    Article  CAS  Google Scholar 

  12. Rasmi RR, Shenoy KB, Sarnaik J, Kadwad VB, Somashekarappa HM, Sivaprasad N (2014) J Radioanal Nucl Chem 302:1271–1275

    Article  CAS  Google Scholar 

  13. Hasson SS, Al-Balushi MS, Al-Jabri AA (2012) Asian Pac J Trop Dis 2:159–162

    Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to Dr. Vijay Kadwad, Sr. Manager, RPHP, BRIT, Mumbai for the supply of in-house produced magnetizable cellulose iron oxide particles.

Author information

Authors and Affiliations

  1. Radiation Medicine Centre, Bhabha Atomic Research Centre (BARC), TMH Annexe, Parel, Mumbai, 400 012, India

    Chandrakala Gholve, Sharmila Banerjee & Savita Kulkarni

Authors
  1. Chandrakala Gholve
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sharmila Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Savita Kulkarni
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Savita Kulkarni.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gholve, C., Banerjee, S. & Kulkarni, S. Novel radioassay for anti-thyroperoxidase autoantibodies using protein A coupled magnetizable cellulose particles as an immunoadsorbent. J Radioanal Nucl Chem 323, 1041–1046 (2020). https://doi.org/10.1007/s10967-020-07023-w

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-020-07023-w

Keywords

Search

Navigation