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Microarray immunoassay for thyrotropin on track-etched membranes using radiotracers

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Abstract

We report a microarray immunoassay for thyrotropin in serum using an anti-thyrotropin monoclonal ‘antibody-chip’ fabricated on polycarbonate track-etched membranes as a solid-support. The thyrotropin microarray immunoassay is an immunoradiometric assay using 125I tracer and requires very small volumes of sample and reagents. The microarray immunoassay had the required minimum detection level, intra- and inter-assay CV and working range to be clinically useful. It correlated very significantly with commercial immunoradiometric assays for thyrotropin. We developed this microarray immunoassay for thyrotropin since it is often used as the first line test for thyroid function.

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Acknowledgements

The authors acknowledge the support from Dr. Deepak Modi, NIRRH, for allowing us to use Phosphorimager, at their institute.

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Authors and Affiliations

  1. Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital Annexe Building, Parel, Mumbai, 400012, India

    Bharti Jain, Savita Kulkarni, Sharmila Banerjee & M. G. Ramakrishna Rajan

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  1. Bharti Jain
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  2. Savita Kulkarni
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  3. Sharmila Banerjee
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  4. M. G. Ramakrishna Rajan
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Correspondence to M. G. Ramakrishna Rajan.

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Jain, B., Kulkarni, S., Banerjee, S. et al. Microarray immunoassay for thyrotropin on track-etched membranes using radiotracers. J Radioanal Nucl Chem 322, 99–104 (2019). https://doi.org/10.1007/s10967-019-06507-8

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  • DOI: https://doi.org/10.1007/s10967-019-06507-8

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