Abstract
In present study, biosynthesis of Au–Ag alloy nanoparticles (NPs) using Commelina nudiflora aqueous extract as a stabilizing and reducing agent is reported. The crystalline nature, size, shape and composition of synthesized Au–Ag alloy NPs were characterized by UV–Vis spectrophotometer, field emission scanning electron spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscope, X-ray diffraction and fourier transform-infrared spectroscopy (FT-IR). The synthesized Au–Ag alloy NPs exhibited different ranges of sizes between 20 and 80 nm with different morphology such as spherical, rod and triangular. FT-IR spectral data revealed that the plant extract contains amine, alcohol, phenol and alkane molecules which are critically involved in the formation of Au–Ag alloy NPs. Finally, the biosynthesized Au–Ag alloys NPs exhibited a strong minimum inhibitory, minimum bactericidal activity against selected oral pathogenic bacteria. The present study gives an important suggestion on plant extract mediated synthesis bimetallic NPs (Au–Ag alloy) emphasis on oral pathogenic bacteria activities.
Similar content being viewed by others
References
D. Dwivedi, K. Gopal, Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids Surf. A 369, 27–33 (2010)
L. Lin, W. Wang, J. Huang, Q. Li, D. Sun, X. Yang, H. Wang, N. He, Y. Wang, Nature factory of silver nanowires: Plant mediated synthesis using broth of Cassia fiatula leaf. Chem. Eng. J. 162, 852–858 (2010)
P.P. Gan, S.H. Ng, Y. Huang, S.F. Li, Green synthesis of gold nanoparticles using palm oil mill effluent (POME): a low-cost and eco-friendly viable approach. Bioresour. Technol. 113, 132–135 (2012)
D.S. Sheny, J. Mathew, D. Philip, Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale. Spectrochim. Acta Part A 79, 254–262 (2011)
C. Tamuly, M. Hazarika,S.Ch. Borah, M.R. Das, M.P. Boruah, In situ biosynthesis of Ag, Au and bimetallic nanoparticles using Piper pedicellatum C.DC: green chemistry approach. Colloids Surf. B 102, 627–634 (2013)
S.S. Shankar, A. Ahmad, R. Pasricha, M. Sastry, Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J. Mater. Chem. 13, 1822–1826 (2004)
G. Wei, H. Zhou, Z. Liu, Y. Song, L. Wang, L. Sun, Z. Li, One-step synthesis of silver nanoparticles, nanorods, and nanowires on the surface of DNA network. J. Phys. Chem. B 109, 8738–8743 (2005)
V. Smuleac, R. Varma, S. Sikdar, D. Bhattacharyya, Green synthesis of Fe and Fe/Pd bimetallic nanoparticles in membrane for degradation of chlorinated organics. J. Memb. Sci. 379, 131–137 (2011)
S. Senapati, A. Syed, S. Moeez, A. Kumar, A. Ahmad, Intracellular synthesis of gold nanoparticles using alga Tetraselmis kochinensis. Mater. Lett. 79, 116–118 (2012)
A.A. Abdelhamid, M.A. Al-Ghobashy, M. Fawzy, M.B. Mohamed, M.M.S.A. Abdel-Mottaleb, Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract of sago pondweed (Potamogeton pectinatus L.). ACS Sustain. Chem. Eng. 1, 1520–1529 (2013)
M.M. Kumari, J. Jacob, D. Philip, Green synthesis and applications of Au–Ag bimetallic nanoparticles. Spectrochim. Acta Part A 137, 185–192 (2015)
S. Yallappa, J. Manjanna, B.L. Dhananjaya, Phytosynthesis of stable Au, Ag and Au–Ag alloy nanoparticles using J. sambac leaves extract, and their enhanced antimicrobial activity in presence of organic antimicrobials. Spectrochim. Acta Part A 137, 236–243 (2015)
Y. Zhang, G. Gao, Q. Qian, D. Cui, Chloroplasts-mediated biosynthesis of nanoscale Au–Ag alloy for 2-butanone assay based on electrochemical sensor. Nanoscale Res. Lett. 7, 475–482 (2012)
J.Y. Song, B.S. Kim, Biological synthesis of bimetallic Au/Ag nanoparticles using Persimmon (Diopyros kaki) leaf extract. Korean J. Chem. Eng 25, 808–811 (2008)
L. **, L. Shengli, Z. Miaotao, Z. Wenlong, L. Chuanghong, Evaluations of antibacterial activity and cytotoxicity on ag nanoparticles evaluations of antibacterial activity and cytotoxicity on Ag nanoparticles. Rare Metal Mater. Eng. 40, 209–214 (2011)
J.Y. Song, B.S. Kim, Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosys. Eng. 32, 79–84 (2009)
H. Liu, W. Tian, B. Li, G. Wu, M. Ibrahim, Z. Tao, Y. Wang, G. **e, H. Li, G. Sun, Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen Rhizoctonia solani. Biotechnol. Lett. 12, 342291–342298 (2012)
H. Mirzaei, M. Darroudi. Zinc oxide nanoparticles: biological synthesis and biomedical applications. Ceram. Int. 43, 907–914 (2017)
F.A.C. Andrade, de L.C.O. Vercik, F.J. Monteiro, da E.C.S. Rigo. Preparation, characterization and antibacterial properties of silver nanoparticles–hydroxyapatite composites by a simple and eco-friendly method. Ceram. Int. 42, 2271–2280 (2016)
A.R. Binupriya, M. Sathishkumar, S.I. Yun, Biocrystallization of silver and gold ions by inactive cell filterate of Rhizopus stolonifer. Colloids. Surf. A 79, 531–534 (2011)
C.O. Ujowundu, C.U. Igwe, V.H.A. Enemor, L.A. Nwaogu, O.E. Okafor, Nutritive and antinutritive effect of Commelina nudiflora plant extract. Pak. J. Nutr. 7, 90–92 (2008)
D. Philip, Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract. Spectrochim. Acta A 73, 374–381 (2009)
P. Raveendran, J. Fu, S.L. Wallen, A simple and “green” method for the synthesis of Au, Ag, and Au–Ag alloy nanoparticles. Green Chem. 8, 34–39 (2006)
P. Kuppusamy, M.M. Yusoff, N.R. Parine, N. Govindan, Evaluation of in-vitro antioxidant and antibacterial properties of Commelina nudiflora L. extracts prepared by different polar solvents. Saudi J. Biol. Sci. 22, 293–301 (2015)
S. Mondal, N. Roy, R.A. Laskar, I. Sk, S. Basu, D. Mandal, N.A. Begum, Biogenic synthesis of Ag, Au and bimetallic Au–Ag alloy nanoparticles using aqueous extract of mahogany (Swietenia mahogany JACQ.) leaves. Colloids Surf. B 82, 497–504 (2011)
M. Moshfegh, H. Forootanfar, B.B. Zare, A.R. Shahverdi, G. Zarrini, M.A. Faramarzi, Biological synthesis of au, ag and au–ag bimetallic nanoparticles by α-amylase. Dig. J. Nanomater Bios. 6, 1419–1426 (2011)
D.E. Gheorghe, L. Cui, C. Karmonik, A. Brazdeikis, J.M. Penaloza, J.K. Young, R.A. Drezek, M. Bikram, Gold–silver alloy nanoshells: a new candidate for nanotherapeutics and diagnostics. Nanoscale Res. Lett. 6, 554–566 (2011)
G. Zhang, M. Du, Q. Li, X. Li, J. Huang, X. Jiang, D. Sun, Green synthesis of Au–Ag alloy nanoparticles using Cacumen platycladi extract. RSC Adv. 3, 1878–1884 (2013)
L. Sun, W. Luan, Y.J. Shan, Au–Ag gradient alloy nanoparticles with extended surface plasmon resonance wavelength: synthesis via microreaction. Nano Biomed. Eng. 3, 227–231 (2012)
S.W. Han, Y. Kim, K. Kim, Dodecanethiol-derivatized Au/Ag bimetallic nanoparticles: TEM, UV/VIS, XPS, and FTIR analysis. J. Colloid Interface Sci. 208, 272–278 (1998)
X. Zhao, T. Toyooka, Y. Ibuki, Synergistic bactericidal effect by combined exposure to Ag nanoparticles and UVA. Sci. Total Environ. 460, 54–62 (2013)
M.T. Zamora-Mendoza, J.L. López-Miranda, G. Rosas, A green approach for self-assembly of Ag–Au nanoparticles into 3-D arrays. Mater. Lett. 186, 311–313 (2017)
Clinical and Laboratory Standards Institute, M100–S22. Performance Standards for Antimicrobial Susceptibility Testing: 22nd Informational Supplement. (CLSI, Wayne, PA, 2012)
V. Vilas, D. Philip, J. Mathew, Biosynthesis of Au and Au/Ag alloy nanoparticles using Coleus aromaticus essential oil and evaluation of their catalytic, antibacterial and antiradical activities. J. Mol. Liq. 221, 179–189 (2016)
J.S. Kim, E. Kuk, K.N. Yu, J.H. Kim, S.J. Park, H.J. Lee, S.H. Kim, Y.K. Park, Y.H. Park, C.Y. Hwang, Y.K. Kim, Y.S. Lee, D.H. Jeong, M.H. Cho, Antimicrobial effects of silver nanoparticles, Nanomedicine 3(1), 95–101 (2007)
I. Sondi, B. Salopek-Sondi, Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 275, 177–182 (2004)
Acknowledgements
This work was carried out with the support of ‘Cooperative Research Program for Agriculture Science & Technology Development [Project title: Technical development to increase the utilization of Italian ryegrass in livestock; Project No. PJ 010903022016’, Rural Development Administration, Republic of Korea]. This study was supported by Postdoctoral Fellowship Program of [National Institute of Animal Science], Rural Development Administration, Republic of Korea.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
None.
Rights and permissions
About this article
Cite this article
Kuppusamy, P., Ilavenil, S., Srigopalram, S. et al. Synthesis of Bimetallic Nanoparticles (Au–Ag Alloy) Using Commelina nudiflora L. Plant Extract and Study its on Oral Pathogenic Bacteria. J Inorg Organomet Polym 27, 562–568 (2017). https://doi.org/10.1007/s10904-017-0498-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10904-017-0498-8