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BODIPY Based OFF-ON Fluorescent Probe for Endogenous Carbon Monoxide Imaging in Living Cells

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Abstract

Carbon monoxide (CO) is one of the signaling molecules that are ubiquitous in humans, which involves in the regulation of human physiology and pathology. In this work, the probe PEC was designed and synthesized based on BODIPY fluorophore that can selectively detect CO through reducing the nitro group to amino group, resulting in a “turn-on” fluorescence response with a simultaneous increase in the concentration of CO. The response is selective over a variety of relevant reactive free radicals, ions, and amino acid species. PEC has the advantages of good stability, good water solubility, and obvious changes in fluorescence signals. In addition, PEC can be used to detect and track endogenous CO in living cells.

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Supplementary material related to this article can be found in the online version.

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Funding

This work was funded by the National Natural Science Foundation of China (21872095), “111” Innovation and Talent Recruitment Base on Photochemical and Energy Materials (No. D18020), Shanghai Engineering Research Center of Green Energy Chemical Engineering (No. 18DZ2254200).

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Authors and Affiliations

  1. The Education Ministry Key Lab of Resource Chemistry, Shanghai Frontiers Science Center of Biomimetic Catalysis, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China

    Lei Zhao, Rui Chen, Cheng Jia, Jiandong Liu, Guohua Liu & Tanyu Cheng

Authors
  1. Lei Zhao
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  2. Rui Chen
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  3. Cheng Jia
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  4. Jiandong Liu
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  5. Guohua Liu
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  6. Tanyu Cheng
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Contributions

Lei Zhao synthesized the probe and mesured the spectra properites. Rui Chen and Cheng Jia conducted cell imagings. Jiandong Liu prepared the figures. Guohua Liu revised the manuscript. Tanyu cheng wrote and revised the manuscript. All authors reviewed the manuscript.

Corresponding author

Correspondence to Tanyu Cheng.

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Zhao, L., Chen, R., Jia, C. et al. BODIPY Based OFF-ON Fluorescent Probe for Endogenous Carbon Monoxide Imaging in Living Cells. J Fluoresc 34, 1793–1799 (2024). https://doi.org/10.1007/s10895-023-03403-z

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